DNA origami
DNA origami
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DOI：
10.1038/s43586-020-00009-8
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发表时间：
2021-01-28
期刊：
影响因子：
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通讯作者：
Zhan, Pengfei
中科院分区：
文献类型：
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作者：
Dey, Swarup;Fan, Chunhai;Zhan, Pengfei
生物材料在活细胞中以接近原子的精度自组装，而合成的3D结构通常缺乏这种精度和可控性。最近，DNA纳米技术，特别是DNA折纸技术，已经在从几十纳米到亚微米范围内的定义明确的纳米结构的自下而上的制造中有用。在这本《入门》中，我们总结了DNA折纸技术的方法论，包括折纸设计、合成、功能化和表征。我们强调折纸结构在纳米制造，纳米光子学和纳米电子学，催化，计算，分子机器，生物成像，药物输送和生物物理学中的应用。我们确定了该领域的挑战，包括尺寸限制，稳定性问题和生产规模，并讨论了可能的解决方案。我们进一步提供了下一代DNA折纸技术，将允许在体内合成和多尺度制造的前景。
Biological materials are self-assembled with near-atomic precision in living cells, whereas synthetic 3D structures generally lack such precision and controllability. Recently, DNA nanotechnology, especially DNA origami technology, has been useful in the bottom-up fabrication of well-defined nanostructures ranging from tens of nanometres to sub-micrometres. In this Primer, we summarize the methodologies of DNA origami technology, including origami design, synthesis, functionalization and characterization. We highlight applications of origami structures in nanofabrication, nanophotonics and nanoelectronics, catalysis, computation, molecular machines, bioimaging, drug delivery and biophysics. We identify challenges for the field, including size limits, stability issues and the scale of production, and discuss their possible solutions. We further provide an outlook on next-generation DNA origami techniques that will allow in vivo synthesis and multiscale manufacturing.