Superoxide dismutases, SOD1 and SOD2, play a distinct role in the fat body during pupation in silkworm Bombyx mori.
Superoxide dismutases, SOD1 and SOD2, play a distinct role in the fat body during pupation in silkworm Bombyx mori.
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DOI：
10.1371/journal.pone.0116007
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Fugo H
中科院分区：
文献类型：
--
作者：
Nojima Y;Ito K;Ono H;Nakazato T;Bono H;Yokoyama T;Sato R;Suetsugu Y;Nakamura Y;Yamamoto K;Satoh J;Tabunoki H;Fugo H
有氧生物系统抵消活性氧 (ROS) 产生的一种方法是使用超氧化物歧化酶蛋白 SOD1 和 SOD2，将超氧自由基代谢为分子氧和过氧化氢，或清除线粒体中发生的广泛氧化还原和电子传递反应产生的氧自由基。我们对从幼虫脂肪体中分离出的家蚕 SOD1 和 SOD2 进行了表征。免疫学分析表明，从幼虫到成虫的丝腺、中肠、脂肪体、马氏小管、睾丸和卵巢中都存在BmSOD1和BmSOD2。我们发现 BmSOD2 在第五龄幼虫发育阶段的脂肪体内具有独特的表达模式。通过将幼虫暴露于杀虫剂鱼藤酮或血管扩张剂二硝酸异山梨酯（BmN4 细胞中的 ROS 发生器）来评估 BmSOD1 和 BmSOD2 的抗氧化功能；然而，暴露于这些化合物对任一 BmSOD 蛋白的表达水平没有影响。接下来，我们研究了 BmSOD1 和 BmSOD2 在环境氧化应激下的生理作用，通过全身紫外线照射，并使用定量 RT-PCR、免疫印迹和微阵列分析进行测定。 BmSOD1和BmSOD2的mRNA表达水平均显着增加，但蛋白表达水平仅略有增加。为了检查由于紫外线照射强度导致的 mRNA 和蛋白质水平的差异，我们进行了微阵列分析。基因集富集分析显示，紫外线照射6小时和12小时后，胰岛素信号通路和PPAR信号通路中的基因显着上调。综上所述，BmSOD1和BmSOD2的活性可能与家蚕对紫外线照射应激的反应有关。这些结果表明BmSOD1和BmSOD2调节细胞内的环境氧化应激，并且在家蚕化蛹期间的脂肪体中具有特定作用。
One way that aerobic biological systems counteract the generation of reactive oxygen species (ROS) is with superoxide dismutase proteins SOD1 and SOD2 that metabolize superoxide radicals to molecular oxygen and hydrogen peroxide or scavenge oxygen radicals produced by the extensive oxidation-reduction and electron-transport reactions that occur in mitochondria. We characterized SOD1 and SOD2 of Bombyx mori isolated from the fat body of larvae. Immunological analysis demonstrated the presence of BmSOD1 and BmSOD2 in the silk gland, midgut, fat body, Malpighian tubules, testis and ovary from larvae to adults. We found that BmSOD2 had a unique expression pattern in the fat body through the fifth instar larval developmental stage. The anti-oxidative functions of BmSOD1 and BmSOD2 were assessed by exposing larvae to insecticide rotenone or vasodilator isosorbide dinitrate, which is an ROS generator in BmN4 cells; however, exposure to these compounds had no effect on the expression levels of either BmSOD protein. Next, we investigated the physiological role of BmSOD1 and BmSOD2 under environmental oxidative stress, applied through whole-body UV irradiation and assayed using quantitative RT-PCR, immunoblotting and microarray analysis. The mRNA expression level of both BmSOD1 and BmSOD2 was markedly increased but protein expression level was increased only slightly. To examine the differences in mRNA and protein level due to UV irradiation intensity, we performed microarray analysis. Gene set enrichment analysis revealed that genes in the insulin signaling pathway and PPAR signaling pathway were significantly up-regulated after 6 and 12 hours of UV irradiation. Taken together, the activities of BmSOD1 and BmSOD2 may be related to the response to UV irradiation stress in B. mori. These results suggest that BmSOD1 and BmSOD2 modulate environmental oxidative stress in the cell and have a specific role in fat body of B. mori during pupation.
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DOI：
10.1073/pnas.78.11.6858
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
AMES, BN;CATHCART, R;HOCHSTEIN, P
通讯作者：
HOCHSTEIN, P
影响因子：
2.6
作者：
Muramatsu, Daisuke;Kinjoh, Terunori;Hiruma, Kiyoshi
通讯作者：
Hiruma, Kiyoshi
影响因子：
64.8
作者：
HAYASHI, Y
通讯作者：
HAYASHI, Y
DOI：
10.1073/pnas.0136976100
发表时间：
2003-01-07
影响因子：
11.1
作者：
Mockett, RJ;Radyuk, SN;Sohal, RS
通讯作者：
Sohal, RS
影响因子：
3.5
作者：
Miller AF
通讯作者：
Miller AF