Human monoclonal antibodies: methods of production and some aspects of their application in oncology.
Human monoclonal antibodies: methods of production and some aspects of their application in oncology.
复制标题
人单克隆抗体：生产方法及其在肿瘤学中应用的某些方面。
DOI：
10.1007/bf02934529
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Olsson,L
中科院分区：
文献类型：
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作者：
Olsson,L
目前可用于生产人单克隆抗体（Mab）的方法有：小鼠-人杂交、人-人杂交、EBV转化以及EBV转化的特异性B淋巴细胞与恶性细胞系的融合。每种方法都有其优点和缺点，但它们都不如最佳功能的小鼠杂交瘤系统。一个重要的障碍是缺乏获得大量抗原特异性B淋巴细胞的可重复方法。然而，新发展的体外抗原引发方法似乎对解决这一问题有重要的帮助。已经获得了针对某些类型的人类癌症的人单克隆抗体，并且反应模式提供了一些非人单克隆抗体似乎无法获得的生物学信息。此外，生产人类单克隆抗体的技术似乎已经发展到这样一个阶段，即它允许一个现实的希望，即它们很快就可以应用于人类癌症的诊断和治疗。
The currently available methods for the production of human monoclonal antibodies (Mabs) are: mouse-human hybrids, human-human hybrids, EBV transformation, and fusion of EBV-transformed specific B-lymphocytes with a malignant cell line. Each method has its advantages and drawbacks, but they are all inferior to an optimal functioning mouse hybridoma system. A significant obstacle is the lack of a reproducible method to obtain high amounts of antigen-specific B-lymphocytes. However, it seems that newly developedin vitroantigen-priming methods may be a significant aid to the solution of this problem.Human Mabs have already been obtained against some types of human cancer, and the reactivity pattern has provided some biological information that seems not to be obtainable with non-human Mabs. It furthermore seems that the technology for production of human Mabs now has progressed to the stage where it permits a realistic hope that they soon can be applied in the diagnosis and treatment of human cancer.