Recombinant conotoxin, TxVIA, produced in yeast has insecticidal activity
Recombinant conotoxin, TxVIA, produced in yeast has insecticidal activity
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DOI：
10.1016/j.toxicon.2011.05.009
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发表时间：
2011-07-01
期刊：
影响因子：
2.8
通讯作者：
Gatehouse, J. A.
中科院分区：
文献类型：
--
作者：
Bruce, C.;Fitches, E. C.;Gatehouse, J. A.
圆锥螺毒素是由圆锥螺属的掠食性海螺产生的超过50,000多肽的多样化集合，目的是使其猎物静止不动。许多concontoxins调节离子通道的活性，并表现出高度特异性的目标；因此，其中一些具有宝贵的制药应用价值。然而，获得活性肽是困难的，迄今为止只能通过自然收集，化学合成或使用原核表达系统来实现，这些系统通常具有需要后续步骤才能正确折叠肽的缺点。本文报道了以毕赤酵母为表达宿主，从Conus纺织品中制备具有生物活性的concontoxin重组蛋白TxVIA。研究发现，前肽的存在对生物活性螺毒素的表达是必要的。我们还发现，TxVIA不像以前报道的那样是软体动物特有的，但当注射到鳞翅目（菜蛾）和双翅目（家蝇）幼虫时，也显示出杀虫活性。重组TxVIA对灰田蛞蝓（Deroceras reticulatum）无杀螺作用。(C) 2011 Elsevier Ltd.版权所有。
Conotoxins are a diverse collection of more than 50,000 peptides produced by predatory marine snails of the genus Conus in order to immobilize their prey. Many conotoxins modulate the activity of ion channels, and show high specificity to their targets; as a result, some have valuable pharmaceutical applications. However, obtaining active peptide is difficult and to date has only been achieved though natural collection, chemical synthesis, or the use of prokaryotic expression systems, which often have the disadvantage of requiring subsequent steps to correctly fold the peptide. This paper reports the production of a conotoxin, TxVIA from Conus textile, as a biologically active recombinant protein, using the yeast Pichia pastoris as expression host. The presence of the pro-peptide was found to be necessary for the expression of biologically active conotoxin. We also show that TxVIA is not, as previously reported, mollusc-specific, but also shows insecticidal activity when injected into lepidopteran (cabbage moth) and dipteran (house fly) larvae. In contrast, recombinant TxVIA was not found to be molluscicidal to the grey field slug Deroceras reticulatum. (C) 2011 Elsevier Ltd. All rights reserved.