Identification of a complete Cek7 receptor protein tyrosine kinase coding sequence and cDNAs of alternatively spliced transcripts.
Identification of a complete Cek7 receptor protein tyrosine kinase coding sequence and cDNAs of alternatively spliced transcripts.
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鉴定完整的 Cek7 受体蛋白酪氨酸激酶编码序列和选择性剪接转录物的 cDNA。
DOI：
10.1016/0378-1119(94)90692-0
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发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
Verderame,MF
中科院分区：
文献类型：
--
作者：
Siever,DA;Verderame,MF
受体蛋白酪氨酸激酶（RPTK）是多细胞生物信号转导途径的重要组成部分。识别新的RPTK构成了理解这些蛋白质参与的各种信号通路的第一步。在这项研究中，含有Cek 7（鸡RPTK）的完整编码序列的cDNA已克隆从鸡胚胎表达文库使用抗磷酸酪氨酸抗体（Ab）。Cek 7是RPTK的EPH（人RPTK）亚家族的成员;该亚家族的特征在于含有免疫球蛋白样基序、富含Cys的区域和两个纤连蛋白III型重复的细胞外结构域。额外的cDNA的分析表明，在初级转录的选择性剪接的两个位置可能会产生几个亚型的RPTK; cDNA对应于三个亚型的这种受体的报告。预计这些亚型具有改变的细胞外配体结合结构域和/或改变的细胞质质膜区域。本研究中鉴定的cek 7 cDNA的核苷酸序列在3'端与最近描述的部分cek 7 cDNA中发现的序列不同[Sajjadi和Pasquale，Oncogene 8（1993）1807-1813]。因此，选择性剪接的第三个位置可以产生具有不同C末端尾部的Cek 7 RPTK。RNA印迹分析显示，这种受体的表达在中枢神经系统和10天大的鸡胚的眼睛在最高水平。
Receptor protein tyrosine kinases (RPTK) are critical components of signal transduction pathways in multicellular organisms. Identification of new RPTK constitutes an initial step in understanding the variety of signalling pathways in which these proteins participate. In this study, a cDNA containing a complete coding sequence for Cek7 (chicken RPTK) has been cloned from a chicken embryo expression library using anti-phosphotyrosine antibodies (Ab). Cek7 is a member of the EPH (human RPTK) subfamily of RPTK; this subfamily is characterized by extracellular domains containing an immunoglobulin-like motif, a Cys-rich region and two fibronectin type-III repeats. Analysis of additional cDNAs revealed that two positions of alternative splicing in primary transcripts may produce several isoforms of this RPTK; cDNAs corresponding to three isoforms of this receptor are reported. These isoforms are predicted to have altered extracellular ligand-binding domains and/or altered cytoplasmic juxtamembrane regions. The nucleotide sequence ofcek7cDNAs identified in this study diverges at the 3' end from the sequence found in a recently described partialcek7cDNA [Sajjadi and Pasquale, Oncogene 8 (1993) 1807-1813]. Therefore, a third position of alternative splicing may produce Cek7 RPTK with divergent C-terminal tails. RNA blot analysis revealed expression of this receptor at highest levels in the central nervous system and eyes of 10-day-old chicken embryos.