Possible correlation between iron deposition and enhanced proliferating activity in hepatitis C virus-positive hepatocellular carcinoma in Myanmar (Burma)
Possible correlation between iron deposition and enhanced proliferating activity in hepatitis C virus-positive hepatocellular carcinoma in Myanmar (Burma)
复制标题
缅甸丙型肝炎病毒阳性肝细胞癌中铁沉积与增殖活性增强之间可能存在相关性
DOI：
10.1007/s00535-006-1993-9
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发表时间：
2007
影响因子：
6.3
通讯作者：
T. Koji
中科院分区：
文献类型：
--
作者：
Kyaw Soe;Y. Hishikawa;Y. Fukuzawa;N. Win;Khine San Yin;K. Win;A. Myint;T. Koji
研究背景本研究的目的是观察在缅甸(缅甸)丙型肝炎病毒(HCV)阳性的肝细胞癌(HCC)患者中铁沉积对细胞动力学的影响。普鲁士蓝染色检测铁沉积。分别用Ki-67染色和末端脱氧核苷酸转移酶(TdT)介导的dUTP-生物素缺口末端标记法(TUNEL)检测细胞增殖和凋亡。原位杂交法检测丙型肝炎病毒核糖核酸，免疫组织化学法检测丙型肝炎病毒蛋白、Fas及Fas配体(FasL)的表达。结果43%的肝细胞癌组织中有铁沉积，且中分化肝细胞癌的铁沉积程度高于高分化肝细胞癌。普鲁士蓝阳性组癌细胞Ki-67标记指数(LI)明显高于阴性组(3.80±2.2vs1.5±1.7，P=0.0067)，而TUNEL标记指数差异无统计学意义(P>0.05)。丙型肝炎病毒蛋白定位于癌细胞，89%的患者体内有丙型肝炎病毒蛋白表达。此外，Fas在肝癌细胞中有表达，FasL在肝癌细胞中有表达，在浸润性CD8+T淋巴细胞中也有表达。结论在缅甸肝癌患者中，铁沉积可能通过促进癌细胞的增殖而加速肝癌的发生，而不影响Fas/FasL的凋亡系统。
BackgroundThe aim of this study was to survey the effect of deposited iron on the cell kinetics of hepatitis C virus (HCV)-positive hepatocellular carcinoma (HCC) in Myanmar (Burmese) patients.MethodsFormalin-fixed and paraffin-embedded liver tissues from 34 Myanmar patients with HCC were used. To detect iron deposition, Prussian blue staining was performed. Cell proliferation and apoptosis were assessed by Ki-67 staining and by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) assay, respectively. HCV RNA was detected by in situ hybridization, and HCV protein, Fas and Fas ligand (FasL) were localized by immunohistochemistry. To identify the subtype of lymphocytes, CD8 was used as a surface marker.ResultsIron deposition was found in 43% of the HCC cases, and was heavier in moderately differentiated HCC than in well-differentiated HCC. The Ki-67 labeling index (LI) in cancer cells was higher in Prussian blue-positive-HCC than in -negative HCC (3.8 ± 2.2 vs 1.5 ± 1.7, mean ± SD; P = 0.0067), whereas there was no significant difference between these groups in TUNEL LI. HCV protein was localized in cancer cells, and was found in 89% of the patients. In addition, Fas was expressed in HCC cells, and FasL was localized in HCC cells as well as in infiltrating CD8+ T lymphocytes. The frequency of apoptosis of HCC cells was correlated significantly with the population density of infiltrating CD8+ T lymphocytes.ConclusionsOur results indicated that, in Myanmar patients with HCC, iron deposition might accelerate hepatocarcinogenesis, by promoting cancer cell proliferation, without affecting the Fas/FasL apoptotic system.
影响因子：
4.8
作者：
Takehiko Koji;R. Brenner
通讯作者：
Takehiko Koji;R. Brenner
影响因子：
29.4
作者：
Kowdley, KV
通讯作者：
Kowdley, KV