Persistent expression of MNF identifies myogenic stem cells in postnatal muscles
Persistent expression of MNF identifies myogenic stem cells in postnatal muscles
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DOI：
10.1006/dbio.1997.8657
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发表时间：
1997-08-15
影响因子：
2.7
通讯作者：
Williams, RS
中科院分区：
文献类型：
--
作者：
Garry, DJ;Yang, QA;Williams, RS
骨骼肌含有未分化的肌源性干细胞池（卫星细胞），可以动员再生肌纤维以应对损伤。我们已经确定，翼螺旋转录因子MNF选择性地表达在静止的卫星细胞，不表达已知的肌原性程序的监管机构。在肌肉损伤后，MNF暂时存在于增殖的卫星细胞和再生肌纤维的集中核中，但表达随着这些纤维的成熟而下降，直到只有残留的干细胞池继续表达可检测水平的MNF。MNF也在发育中的肌节、肢芽前体和心管中选择性地但瞬时地表达于肌发生的胚胎阶段，但在发育的晚期胎儿阶段，MNF在分化的心脏和骨骼肌细胞内下调，并且仅在卫星细胞中观察到持续的高表达。这些数据确定MNF作为静止卫星细胞的标志物，并表明由MNF控制的下游基因用于调节这种独特的细胞群中的增殖生长或分化。(C)北京：科学出版社.
Skeletal muscles contain an undifferentiated myogenic stem cell pool (satellite cells) that can be mobilized to regenerate myofibers in response to injury. We have determined that the winged helix transcription factor MNF is expressed selectively in quiescent satellite cells, which do not express known regulators of the myogenic program. Following muscle injury, MNF is present transiently in proliferating satellite cells and in centralized nuclei of regenerating myofibers, but expression declines as these fibers mature, until only the residual stem cell pool continues to express detectable levels of MNF. MNF also is expressed selectively but transiently at embryonic stages of myogenesis in the developing myotome, limb bud precursors, and heart tube, but by late fetal stages of development, MNF is down-regulated within differentiated cardiac and skeletal myocytes, and persistently high expression is observed only in satellite cells. These data identify MNF as a marker of quiescent satellite cells and suggest that downstream genes controlled by MNF serve to modulate proliferative growth or differentiation in this unique cell population. (C) 1997 Academic Press.