Transcriptome analysis of bacteriophage communities in periodontal health and disease.
Transcriptome analysis of bacteriophage communities in periodontal health and disease.
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DOI：
10.1186/s12864-015-1781-0
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发表时间：
2015-07-28
期刊：
影响因子：
4.4
通讯作者：
Pride DT
中科院分区：
文献类型：
--
作者：
Santiago-Rodriguez TM;Naidu M;Abeles SR;Boehm TK;Ly M;Pride DT
病毒作为人类微生物组成员的作用随着发现人体表面居住着相当大的病毒群落而获得了更广泛的关注。在这些群落中发现的大多数病毒是噬菌体，它们先于细胞微生物群而不是人类宿主。噬菌体具有裂解宿主或通过溶原性转化为宿主提供选择性优势的能力，这有助于确定共存细菌群落的结构。由于牙周炎等疾病与细菌生物群的改变有关，因此已经假设噬菌体介导的细菌群落扰动在促进牙周疾病中起作用。口腔噬菌体群落在牙周健康和疾病之间也有显着差异，但口腔噬菌体群落的基因表达以前没有研究过。在这里，我们提供了第一份报告的基因表达谱从口腔噬菌体社区使用RNA测序，并发现口腔噬菌体更高表达的受试者相对牙周健康。虽然溶素高度表达，整合酶的高比例表达表明，原噬菌体可能占相当大比例的口腔噬菌体基因表达。许多转录组读数与研究受试者口腔中发现的fos相匹配，表明fos可能占口腔基因表达的很大比例。与感染厚壁菌门的虹吸病毒同源的读段是在牙周健康和疾病中鉴定的最普遍的转录组读段之一。来自噬菌体裂解模块的一些基因在患有牙周病的受试者中显著更高地表达，这表明牙周炎可能有利于一些裂解因子的表达。当我们探索病毒对人类微生物组的贡献时，这里提供的数据表明噬菌体群落在口腔健康和疾病中的不同表达。本文的在线版本（doi：10.1186/s12864-015-1781-0）包含补充材料，可供授权用户使用。
The role of viruses as members of the human microbiome has gained broader attention with the discovery that human body surfaces are inhabited by sizeable viral communities. The majority of the viruses identified in these communities have been bacteriophages that predate upon cellular microbiota rather than the human host. Phages have the capacity to lyse their hosts or provide them with selective advantages through lysogenic conversion, which could help determine the structure of co-existing bacterial communities. Because conditions such as periodontitis are associated with altered bacterial biota, phage mediated perturbations of bacterial communities have been hypothesized to play a role in promoting periodontal disease. Oral phage communities also differ significantly between periodontal health and disease, but the gene expression of oral phage communities has not been previously examined. Here, we provide the first report of gene expression profiles from the oral bacteriophage community using RNA sequencing, and find that oral phages are more highly expressed in subjects with relative periodontal health. While lysins were highly expressed, the high proportion of integrases expressed suggests that prophages may account for a considerable proportion of oral phage gene expression. Many of the transcriptome reads matched phages found in the oral cavities of the subjects studied, indicating that phages may account for a substantial proportion of oral gene expression. Reads homologous to siphoviruses that infect Firmicutes were amongst the most prevalent transcriptome reads identified in both periodontal health and disease. Some genes from the phage lytic module were significantly more highly expressed in subjects with periodontal disease, suggesting that periodontitis may favor the expression of some lytic phages. As we explore the contributions of viruses to the human microbiome, the data presented here suggest varying expression of bacteriophage communities in oral health and disease. The online version of this article (doi:10.1186/s12864-015-1781-0) contains supplementary material, which is available to authorized users.