N-Acetylcysteine and deferoxamine reduce pulmonary oxidative stress and inflammation in rats after coal dust exposure
N-Acetylcysteine and deferoxamine reduce pulmonary oxidative stress and inflammation in rats after coal dust exposure
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DOI：
10.1016/j.envres.2005.03.005
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发表时间：
2005-11-01
影响因子：
8.3
通讯作者：
Moreira, JCF
中科院分区：
文献类型：
--
作者：
Pinho, RA;Silveira, PCL;Moreira, JCF
煤尘吸入可引起肺实质氧化损伤和炎症浸润。因此，本研究的目的是确定n -乙酰半胱氨酸（NAC）单独给药或与去铁胺（DFX）联合给药是否能显著减少煤尘暴露大鼠肺部的炎症浸润和氧化损伤。将42只雄性Wistar大鼠（200 ~ 250 g）经气管内灌注煤尘（3 mg/0.5 mL生理盐水，每周3天，连续3周）。随机分为0.9%生理盐水组（n = 8）、添加NAC （20 mg/kg体重/天，腹腔注射）组（n = 8）和添加NAC (20 mg/kg体重/天，i.p) + DFX （20 mg/kg体重/周）组（n = 8）。对照组只给予生理盐水溶液（0.5 m，L）。测定支气管肺泡灌洗液乳酸脱氢酶活性和细胞总数。我们测定了动物肺部脂质过氧化和氧化蛋白损伤参数以及过氧化氢酶和超氧化物歧化酶活性。在大鼠肺内气管内灌注煤尘可引起炎症反应并诱导明显的氧化损伤。NAC单独或与DFX联合使用可降低煤尘暴露大鼠的炎症反应和氧化应激参数。(c) 2005爱思唯尔公司版权所有。
Coal dust inhalation induces oxidative damage and inflammatory infiltration on lung parenchyma. Thus, the aim of this study was to determine whether N-acetylcysteine (NAC) administered alone or in combination with deferoxamine (DFX), significantly reduced the inflammatory infiltration and oxidative damage in the lungs of rats exposed to coal dust. Forty-two male Wistar rats (200-250 g) were exposed to the coal dust (3 mg/0.5 mL saline, 3 days/week, for 3 weeks) by intratracheal instillation. The animals were randomly divided into three groups: saline 0.9% (n = 8), supplemented with NAC (20 mg/kg of body weight/day, intraperitoneal injection (i.p.)) (n = 8), and supplemented with NAC (20 mg/kg of body weight/day, i.p.) plus DFX (20 mg/kg of body weight/week) (n = 8). Control animals received only saline solution (0.5 m,L). Lactate dehydrogenase activity and total cell number were determined in the bronchoalveolar lavage fluid. We determined lipid peroxidation and oxidative protein damage parameters and catalase and superoxide dismutase activities in the lungs of animals. Intratracheal instillation of coal dust in the lungs of rats led to an inflammatory response and induced significant oxidative damage. The administration of NAC alone or in association with DFX reduced the inflammatory response and the oxidative stress parameters in rats exposed to coal dust. (c) 2005 Elsevier Inc. All rights reserved.