Capillary electrophoretic assay for nitrate levels in the vitreous of proliferative diabetic retinopathy
Capillary electrophoretic assay for nitrate levels in the vitreous of proliferative diabetic retinopathy
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DOI：
10.1016/j.jchromb.2006.10.023
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发表时间：
2007-03-01
影响因子：
3
通讯作者：
Shippy, Scott A.
中科院分区：
文献类型：
--
作者：
Gao, Leyi;Pulido, Jose S.;Shippy, Scott A.
人体玻璃体样品中一氧化氮(NO)的测定由于分析物和粘性、高盐、高蛋白质生物基质的半衰期相对较短而变得复杂。在这项工作中，我们建立了一种快速(<；5分钟)和有用的毛细管电泳法来测定玻璃样品中稳定的代谢物硝酸盐。该方法已成功地应用于各种情况下玻璃体切除手术患者玻璃体体液中硝酸盐水平的测定。增殖型糖尿病视网膜病变患者玻璃体中硝酸盐含量(41.17±4.09muM，n=27)明显高于对照组(15.22±0.86muM，n=35)，差异有统计学意义(P=0.000001)。已知的糖尿病视网膜病变患者玻璃体中硝酸盐水平的升高表明NO参与了这种疾病的病理过程。(C)2006爱思唯尔B.V.保留所有权利。
The determination of nitric oxide (NO) in human vitreous samples is complicated by the relatively short half-life of the analyte and the viscous, high salt and protein biological matrix. In this work, we developed a fast (< 5 min) and useful CE method to determine the stable metabolite, nitrate, from vitreous samples. This proposed method has been successfully applied to determine the nitrate levels from the vitreous humor of patients undergoing vitrectomy for a variety of conditions. A statistically significant increase (P=0.000001) of the mean level of nitrate was observed in vitreous humor of patients with proliferative diabetic retinopathy (41.17 +/- 4.09 mu M, n=27) versus controls (15.22 +/- 0.86 mu M, n=35). The elevated levels of nitrate in the vitreous of patients known to have diabetic retinopathy suggests that NO is involved with the pathology of this disease. (c) 2006 Elsevier B.V. All rights reserved.