miR-302b suppresses cell invasion and metastasis by directly targeting AKT2 in human hepatocellular carcinoma cells
miR-302b suppresses cell invasion and metastasis by directly targeting AKT2 in human hepatocellular carcinoma cells
复制标题
miR-302b通过直接靶向人肝癌细胞中的AKT2抑制细胞侵袭和转移
DOI：
10.1007/s13277-015-3330-5
复制
发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Huang, Chen
中科院分区：
文献类型：
--
作者：
Wang, Lumin;Yao, Jiayi;Huang, Chen
MicroRNAs(MiRNAs)在调节人肝细胞癌细胞活性方面发挥重要作用，从而有助于抑制肿瘤的侵袭和转移。在这项研究中，通过功能获得和功能丧失的分析，我们证明miR-302B在临床肝细胞癌组织中经常下调，与15例相应的癌旁正常组织相比。MiR-302B过表达抑制了肝癌细胞的侵袭和转移。MIR-302B通过AKT2调节κ-B和基质金属蛋白酶-2的表达。沉默AKT2的作用类似于miR-302B过表达，包括抑制SMMC-772 1细胞的侵袭和转移，下调NF-κB和MMP2的表达。此外，AKT2的过表达减弱了miR-302B过表达的影响。综上所述，我们的研究结果表明miR-302B通过靶向AKT2抑制SMMC-7721细胞的侵袭和转移，提示miR-302B可能是肝癌干预的潜在治疗靶点。
MicroRNAs (miRNAs) have been shown to play essential roles in regulating the activity of human hepatocellular carcinoma (HCC) cells, thereby contributing to the suppression of invasion and metastasis. In this study, using gain and loss of function assays, we demonstrated that miR-302b was frequently down-regulated in clinical HCC specimens, as compared with 15 corresponding adjacent normal tissues. Overexpression of miR-302b suppressed HCC cell invasion and metastasis. Regulation of NF-κB and matrix metalloproteinase (MMP)-2 expression by miR-302b was mediated via AKT2 in SMMC-7721 cells. Silencing AKT2 produced effects similar to those of miR-302b overexpression, which included inhibiting SMMC-7721 cell invasion and metastasis and dereasing NF-κB and MMP-2 expression. Furthermore, overexpression of AKT2 attenuated the effects of miR-302b overexpression. Taken together, our findings indicate that miR-302b inhibits SMMC-7721 cell invasion and metastasis by targeting AKT2, suggesting that miR-302b might represent a potential therapeutic target for HCC intervention.