D-dimer is an essential accompaniment of circulating tumor cells in gastric cancer.
D-dimer is an essential accompaniment of circulating tumor cells in gastric cancer.
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D-二聚体是胃癌循环肿瘤细胞的重要伴随物
DOI：
10.1186/s12885-016-3043-1
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发表时间：
2017-01-13
期刊：
影响因子：
3.8
通讯作者：
Dang C
中科院分区：
文献类型：
--
作者：
Diao D;Cheng Y;Song Y;Zhang H;Zhou Z;Dang C
纤维蛋白原（FIB）是纤维蛋白的重要来源，在循环肿瘤细胞（CTC）外渗和远处转移的发展中起着至关重要的作用。我们推测其稳定的终产物，血浆D-二聚体，可能与CTC的出现有关，并可以反映癌症患者的转移表型。我们首先在不同的小鼠胃癌转移模型中验证了我们的假设，在三种转移免疫缺陷小鼠模型和对照中直接检测血浆D-二聚体和纤维蛋白原及其降解产物。接下来，我们收集并分析了41例晚期原发性胃癌（GC）患者血浆D-二聚体水平和CTC数量的结果。对这些患者进行了随访研究。在三种体内小鼠转移模型中，与皮下肿瘤模型和对照组相比，血源性和腹膜内转移小鼠模型中血浆D-二聚体水平极高，支持我们先前的假设。41例胃癌患者中，有远处转移者血浆D-二聚体水平明显升高，尤以内脏转移者为甚。血浆D-二聚体水平与肿瘤细胞数呈线性相关（R2 = 0.688，p < 0.001），血浆D-二聚体是较好的生存预测因子。血浆D-二聚体是胃癌中CTC的重要伴随物，具有检测简便、成本低的优点，可用于血行转移的检测。
Fibrinogen (FIB) is an important source of fibrin, which plays a crucial role in circulating tumor cells (CTCs) extravasation and distant metastasis development. We hypothesize it’s stable final product, plasma D-dimer, may be associated with CTCs appearance and can reflect the metastatic phenotype in cancer patients. We first verified our hypothesis in different murine gastric cancer metastasis models in vivo, plasma D-dimer and fibrinogen as well as its degradation products were directly examined in three metastasis immune-deficient mouse models and in controls. Next, we gathered and analyzed the result of plasma D-dimer levels and CTCs numbers in 41 advanced primary gastric cancer (GC) patients. A follow-up study was conducted in these patients. In three in vivo murine metastasis models, plasma D-dimer levels were extremely elevated in a hematogenous and intraperitoneal murine model of metastasis compared with a subcutaneous tumor model and the control group, supporting our previous hypothesis. While in 41 GC patients, the result displayed that plasma D-dimer levels were remarkably increased in patients with distant metastases, especially in visceral metastases patients. Additionally, linear association was shown between D-dimer level and CTCs numbers (R 2 = 0.688, p < 0.001), additionally, plasma D-dimer represent a better survival predictor than CTCs. Plasma D-dimer is an essential accompaniment of CTCs in GC that is easy to measure and lower in cost, and can be used in the detection of hematogenous metastasis.