Occurrence of the Bluefin Tuna Blood Fluke Cardicola opisthorchis in the Intermediate Host Terebella sp.
Occurrence of the Bluefin Tuna Blood Fluke Cardicola opisthorchis in the Intermediate Host Terebella sp.
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DOI：
10.3147/jsfp.50.105
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发表时间：
2015-09-01
期刊：
影响因子：
0.6
通讯作者：
Kanai, Kinya
中科院分区：
文献类型：
--
作者：
Sugihara, Yukitaka;Yamada, Toshiyuki;Kanai, Kinya
后睾吸虫（Cardicola opisthorchis）是日本养殖太平洋金枪鱼（Thunnus orientalis）的重要病原。我们在2014年对中间多毛类宿主Terebellasp.（多毛类：Terebelladae）进行了为期一年的月度调查，以阐明C.日本长崎县对马岛的一个金枪鱼养殖场发生了后睾吸虫感染。中间宿主，收集的绳索和浮子连接到文化笼，是丰富的，从1月至3月，11月和12月，但相当稀缺，从4月至10月。C. 1 ~ 3月和9 ~ 12月发现后睾吸虫，感染率为2.0%~ 18.4%。在中间宿主小盾壳虫体内观察到孢子囊的发育，在多毛类宿主体腔内同时存在生孢子囊和生尾蚴孢子囊。首次详细描述了海洋无孔子叶类孢子囊的无性繁殖过程。此外，孢子囊形成的孢子囊和释放的子孢子囊在L-15培养基中分别存活长达63天和66天。在一个多毛类宿主中，孢子囊的最高数量约为1，800个，并且在一个孢子囊中的尾蚴的最高数量为75个，这表明多达>10（5）个尾蚴可以从一个受感染的多毛类释放。
The blood fluke Cardicola opisthorchis (Trematoda: Aporocotylidae) is a significant pathogen of cultured Pacific bluefin tuna Thunnus orientalis in Japan. We conducted monthly surveys of the intermediate polychaete host Terebella sp. (Polychaeta: Terebellidae) for one year in 2014 to elucidate seasonality in the C. opisthorchis infection at a tuna farm in Tsushima Island, Nagasaki Prefecture, Japan. The intermediate host, collected from ropes and floats attached to culture cages, was abundant from January to March and in November and December but quite scarce from April to October. The sporocysts of C. opisthorchis were found from January to March and from September to December, with the prevalence of infection ranging from 2.0% to 18.4%. Development of sporocysts was observed in the body of the intermediate host Terebella sp. Sporocystogenous sporocysts and cercariogenous sporocysts co-occurred in the body cavities of the polychaete host. This is the first detailed description of asexual multiplication process of sporocysts among marine aporocotylid species. Additionally, sporocystogenous sporocysts and released daughter sporocysts survived in L-15 medium for up to 63 and 66 days, respectively. The highest number of sporocysts in one polychaete host was about 1,800 and the highest number of cercariae in one sporocyst was 75, suggesting that as many as >10(5) cercariae can be released from one infected polychaete.