mRNA phenotyping for studying gene expression in small numbers of cells: platelet-derived growth factor and other growth factors in wound-derived macrophages.
mRNA phenotyping for studying gene expression in small numbers of cells: platelet-derived growth factor and other growth factors in wound-derived macrophages.
复制标题
用于研究少量细胞中基因表达的 mRNA 表型分析：血小板衍生生长因子和伤口衍生巨噬细胞中的其他生长因子。
DOI：
10.1165/ajrcmb/2.1.3
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发表时间：
1990
影响因子：
6.4
通讯作者：
Werb,Z
中科院分区：
文献类型：
--
作者：
Rappolee,DA;Werb,Z
细胞和组织功能的研究需要能够在正常和病理生理条件下测定细胞的分子表型。在肺中，对正常细胞的基因表达以及它们在疾病过程中如何变化知之甚少，因为很难获得多于少量的同质细胞，并且它们在培养中无法增殖。在实验上，优选测定新鲜分离的细胞和组织以避免器官或细胞培养伪影。在临床上，少量组织或细胞的测定允许对细胞生理学进行时间分析。在这次更新中，我们讨论了一种新的分子技术，用于对单个细胞或少量细胞中的基因表达进行表型分析。该技术在肺生物学研究中具有重要应用。应当
The study of cellular and tissue function requires the ability to assay the molecular phenotype of cells under normal and pathophysiologic conditions. In the lung, very little is known about gene expression by normal cells and how they are changed in disease processes because of the difficulty in obtaining more than a small number of homogeneous cells and their failure to proliferate in culture. Experimentally, it is preferable to assay freshly isolated cells and tissues to avoid organ or cell culture artifacts. Clinically, the assay of small amounts of tissues or cells allows the temporal analysis of cellular physiology. In this Update, we discuss a new molecular technique for phenotyping the gene expression in a single cell or small numbers of cells. This technique has important applications for the study of lung biology. It should be