Regulating Oxygen Levels in a Microfluidic Device
Regulating Oxygen Levels in a Microfluidic Device
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DOI：
10.1021/ac202300g
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发表时间：
2011-11-15
影响因子：
7.4
通讯作者：
Forry, Samuel P.
中科院分区：
文献类型：
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作者：
Thomas, Peter C.;Raghavan, Srinivasa R.;Forry, Samuel P.
由聚二甲基硅氧烷 (PDMS) 制成的微流体装置具有透气性，已用于提供精确的片上氧气调节。然而，PDMS 装置中的渗透蒸发会迅速导致溶液渗透压发生巨大变化。在本研究中，我们展示了一种片上氧气控制的新方法，使用气体控制通道中的预平衡水溶液来调节停滞微流体测试室中的氧气含量。片外气体交换器用于在进入芯片之前平衡每种对照溶液。使用这种策略，由于渗透汽化引起的问题大大减少。基于 PDMS 的集成氧气传感器可以准确实时测量微流体室内的氧气。发现测量结果与有限元模型的预测一致。
Microfluidic devices made from poly(dimethylsiloxane) (PDMS) are gas permeable and have been used to provide accurate on-chip oxygen regulation. However, pervaporation in PDMS devices can rapidly lead to dramatic changes in solution osmotic pressure. In the present study, we demonstrate a new method for on-chip oxygen control using pre-equilibrated aqueous solutions in gas-control channels to regulate the oxygen content in stagnant microfluidic test chambers. An off-chip gas exchanger is used to equilibrate each control solution prior to entering the chip. Using this strategy, problems due to pervaporation are considerably reduced. An integrated PDMS-based oxygen sensor allows accurate real-time measurements of the oxygen within the microfluidic chamber. The measurements were found to be consistent with predictions from finite-element modeling.