Solute retention in electrochromatography by electrically induced sorption
Solute retention in electrochromatography by electrically induced sorption
复制标题
DOI：
10.1002/aic.690390508
复制
发表时间：
1993-05
期刊：
影响因子：
3.7
通讯作者：
S. Rudge;S. Basak;M. Ladisch
中科院分区：
文献类型：
--
作者：
S. Rudge;S. Basak;M. Ladisch
柱色谱和电泳在电色谱中结合，其中电势沿轴向方向施加到色谱柱。这些研究利用葡聚糖凝胶固定相和低离子强度的洗脱液，选择它们以使电流最小化，从而使柱加热和不期望的分散效应最小化。该凝胶的离子交换容量很小，为每毫升几个微当量，事实证明比洗脱液更具导电性，并且能够在电场和流场相结合的情况下浓缩大分子。该模型描述了溶质保留由于电诱导的浓度极化的溶质在树脂表面上，以及在移动的和固定相的电泳。极化效应解释了排阻系数小于1的高分子量溶质和带电的低分子量溶质的保留之间的差异，该带电的低分子量溶质假设在电场的存在下穿过凝胶基质并且不经历浓度极化。它也显示了这种效果的应用蛋白质分离的液相色谱系统与叠加的电位。
Column chromatography and electrophoresis are combined in electrochromatography, where an electric potential is applied to a chromatography column in the axial direction. These studies utilized a dextran gel stationary phase and an eluent of low ionic strength, which were chosen to minimize electric current and therefore column heating and undesirable dispersion effects. The gel, with a small ion exchange capacity of several microequivalents per mL, turned out to be more conductive than the eluent and was able to concentrate macromolecules in the presence of combined electric and flow fields. The model presented describes solute retention due to electrically induced concentration polarization of solute on the resin surfaces, as well as electrophoresis in the mobile and stationary phases. The polarization effect explains differences between retention of high-molecular-weight solutes with exclusion coefficients of less than 1 and that of a charged low-molecular-weight solute, which is hypothesized to pass through the gel matrix in the presence of an electric field and does not experience concentration polarization. It also shows the application of this effect for protein separation in a liquid chromatography system with a superimposed electric potential.