Loss of polymorphic restriction fragments in malignant melanoma: implications for tumor heterogeneity.
Loss of polymorphic restriction fragments in malignant melanoma: implications for tumor heterogeneity.
复制标题
恶性黑色素瘤中多态性限制片段的丢失：对肿瘤异质性的影响。
DOI：
10.1073/pnas.82.5.1470
复制
发表时间：
1985
影响因子：
11.1
通讯作者：
Old,LJ
中科院分区：
文献类型：
--
作者：
Dracopoli,NC;Houghton,AN;Old,LJ
某些染色体位点遗传物质的丢失与视网膜母细胞瘤和肾母细胞瘤的病因有关。特定的染色体缺失是否与其他类型的人类癌症有关，还需要探索。我们检查了24个黑色素瘤细胞系，来自21例非家族性恶性黑色素瘤，体细胞诱导的半合子或纯合子的证据。12个DNA探针，识别单拷贝限制性片段长度多态性（RFLP）确定的11个不同的染色体上的位点，被用来筛选自体组合的黑色素瘤细胞和B细胞或成纤维细胞。黑色素瘤细胞中的杂合性丢失在100个信息位点中的27个被鉴定。这些损失发生在8条不同染色体上的基因座上，单个基因座的损失频率在8%和67%之间变化。我们的结论是，体细胞突变导致纯合性或半合子是常见的黑色素瘤，显然不限于特定的染色体。
Loss of genetic material at certain chromosomal sites is implicated in the etiology of retinoblastoma and Wilms tumor. Whether specific chromosomal deletions are associated with other types of human cancer needs to be explored. We have examined 24 melanoma cell lines, derived from 21 patients with nonfamilial malignant melanoma, for evidence of somatically induced hemizygosity or homozygosity. Twelve DNA probes, recognizing single-copy restriction fragment length polymorphisms (RFLP) determined by loci on 11 different chromosomes, were used to screen autologous combinations of melanoma cells and either B cells or fibroblasts. Loss of heterozygosity in melanoma cells was identified at 27 of 100 informative loci. These losses occurred at loci on 8 different chromosomes, and the frequency of loss at individual loci varied between 8% and 67%. We conclude that somatic mutations resulting in homozygosity or hemizygosity are common in melanoma and evidently not restricted to specific chromosomes.