Raman spectroscopy monitoring of the cellular activities of a tissue-engineered ex vivo produced oral mucosal equivalent
Raman spectroscopy monitoring of the cellular activities of a tissue-engineered ex vivo produced oral mucosal equivalent
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DOI：
10.1002/jrs.2688
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发表时间：
2011-02-01
影响因子：
2.5
通讯作者：
Chiang, Huihua Kenny
中科院分区：
文献类型：
--
作者：
Lo, Wen-Liang;Lai, Jian-Yun;Chiang, Huihua Kenny
为了确保组织工程的质量控制和保证，在产品发布之前，需要对基于细胞的设备进行非侵入性、实时和无菌评估。在这项研究中，应用拉曼光谱检测了体外培养的自体口腔角质形成细胞和脱细胞真皮-异体真皮产生的口腔黏膜替代物(EVPOME)的细胞活性。拉曼光谱显示，随着培养天数的增加，酰胺I的峰面积比(1655 cm(-1))/苯丙氨酸(1004 cm(-1))呈负相关(R(2)>；0.95)，特别是在第14天和第21天。这项工作证明了拉曼光谱在定量监测和评价EVPOME成熟度方面的成功应用。版权所有(C)2010 John Wiley&Sons，Ltd.
To ensure quality control and assurance in tissue engineering, noninvasive, real-time and aseptic evaluation of cell-based devices is required before product release. In this study, Raman spectroscopy was applied to monitor the cellular activities of an oral mucosa equivalent (EVPOME) produced ex vivo from cultured autogenous oral keratinocytes and acellular dermis - AlloDerm. Raman spectra showed a positive correlation of the peak area ratio of amide I (1655 cm(-1))/phenylalanine (1004 cm(-1)) with a negative linear regression (R(2) > 0.95) according to the number of cultured days, especially on the 14th and 21st day. This work demonstrates the successful application of Raman spectroscopy for quantitatively monitoring and evaluating the maturity of EVPOME. Copyright (C) 2010 John Wiley & Sons, Ltd.