Effects of phorbol-12-myristate-13-acetate on the phenotypic program of cultured chondroblasts and fibroblasts.
Effects of phorbol-12-myristate-13-acetate on the phenotypic program of cultured chondroblasts and fibroblasts.
复制标题
佛波醇 12-肉豆蔻酸酯-13-乙酸酯对培养的软骨细胞和成纤维细胞表型程序的影响。
作者：
M. Lowe;M. Pacifici;H. Holtzer
佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）对培养的鸡成软骨细胞具有迅速、差异和部分可逆的作用。在36小时内，PMA将无柄、多边形、上皮样成软骨细胞转化为能动的、多层的成纤维细胞。在PMA中，鸡成软骨细胞迅速停止合成其两种末端奢侈分子，即表征细胞外基质的IV型硫酸化蛋白聚糖和表观分子量为180，000的糖基化蛋白。这种糖基化蛋白质约占正常成软骨细胞总蛋白质的5%。如果在PMA中4天后返回到正常培养基，实际上100%的细胞重新开始合成其IV型硫酸化蛋白聚糖，即180，000-道尔顿蛋白，并重新获得其多边形上皮样形态。如果在PMA中12天后返回到正常培养基，则细胞不能合成其两种特征性奢侈分子，并且100%的细胞保持成纤维细胞。PMA改变鸡成纤维细胞的形态，但不阻止其特征性III型硫酸化蛋白聚糖的合成。PMA被证明是成软骨细胞的有丝分裂原，但不是成纤维细胞的有丝分裂原，尽管这两种细胞类型的表型相似。
Phorbol-12-myristate-13-acetate (PMA) has a prompt, differential, and partially reversible effect on cultured chick chondroblasts. Within 36 hr PMA transforms sessile, polygonal, epithelioid chondroblasts into motile, multilayered, fibroblastic cells. In PMA chick chondroblasts rapidly cease to synthesize two of their terminal luxury molecules, the type IV sulfated proteoglycan that characterizes the extracell matrix and a glycosylated protein with an apparent molecular weight of 180,000. This glycosylated protein constitutes approximately 5% of the total protein in normal chondroblasts. If returned to normal medium after 4 days in PMA, virtually 100% of the cells reinitiate the synthesis of their type IV sulfated proteoglycan, of the 180,000-dalton protein, and reacquire their polygonal, epithelioid morphology. If returned to normal medium after 12 days in PMA, the cells fail to synthesize their two characteristic luxury molecules, and 100% of the cells remain fibroblastic. PMA alters the morphology of chick fibroblasts but does not block synthesis of their characteristic type III sulfated proteoglycan. PMA proves to be a mitogen for chondroblasts but not for fibroblasts, in spite of the phenotypic similarities of these two cell types.