Dynamics of enhanced mitochondrial respiration in female compared with male rat cerebral arteries.
Dynamics of enhanced mitochondrial respiration in female compared with male rat cerebral arteries.
复制标题
与雄性大鼠脑动脉相比，雌性大鼠线粒体呼吸增强的动态。
DOI：
10.1152/ajpheart.00231.2015
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Busija,DavidW
中科院分区：
文献类型：
--
作者：
Rutkai,Ibolya;Dutta,Somhrita;Katakam,PrasadV;Busija,DavidW
Mitochondrial respiration has never been directly examined in intact cerebral arteries. We tested the hypothesis that mitochondrial energetics of large cerebral arteries ex vivo are sex dependent. The Seahorse XFe24 analyzer was used to examine mitochondrial respiration in isolated cerebral arteries from adult male and female Sprague-Dawley rats. We examined the role of nitric oxide (NO) on mitochondrial respiration under basal conditions, usingNω-nitro-l-arginine methyl ester, and following pharmacological challenge using diazoxide (DZ), and also determined levels of mitochondrial and nonmitochondrial proteins using Western blot, and vascular diameter responses to DZ. The components of mitochondrial respiration including basal respiration, ATP production, proton leak, maximal respiration, and spare respiratory capacity were elevated in females compared with males, but increased in both male and female arteries in the presence of the NOS inhibitor. Although acute DZ treatment had little effect on mitochondrial respiration of male arteries, it decreased the respiration in female arteries. Levels of mitochondrial proteins in Complexes I–V and the voltage-dependent anion channel protein were elevated in female compared with male cerebral arteries. The DZ-induced vasodilation was greater in females than in males. Our findings show that substantial sex differences in mitochondrial respiratory dynamics exist in large cerebral arteries and may provide the mechanistic basis for observations that the female cerebral vasculature is more adaptable after injury.
