A bio-barcode assay for on-chip attomolar-sensitivity protein detection
A bio-barcode assay for on-chip attomolar-sensitivity protein detection
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DOI：
10.1039/b606294f
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发表时间：
2006-10-01
期刊：
影响因子：
6.1
通讯作者：
Liu, Chang
中科院分区：
文献类型：
--
作者：
Goluch, Edgar D.;Nam, Jwa-Min;Liu, Chang
功能化纳米粒子在实现高灵敏度和高选择性的生物检测方面具有巨大的潜力。我们报告了一个单一的一次性芯片，这是能够进行多步骤的过程中，采用纳米粒子的生物条形码测定（BCA）的单一蛋白质标记物检测。为了说明系统的能力，我们测试了缓冲溶液和山羊血清中前列腺特异性抗原（PSA）的存在。在PSA浓度低至500 aM时完成检测。这对应于使用1 μ L总样品体积仅300个蛋白质分析物拷贝。我们确定，用于PSA检测的片上BCA与市售的基于ELISA的PSA检测相比，灵敏度高出四个数量级。
Functionalized nanoparticles hold great promise in realizing highly sensitive and selective biodetection. We report a single disposable chip which is capable of carrying out a multi-step process that employs nanoparticles-a bio-barcode assay (BCA) for single protein marker detection. To illustrate the capability of the system, we tested for the presence of prostate specific antigen (PSA) in buffer solution and goat serum. Detection was accomplished at PSA concentrations as low as 500 aM. This corresponds to only 300 copies of protein analytes using I mu L total sample volume. We established that the on-chip BCA for PSA detection offers four orders of magnitude higher sensitivity compared to commercially available ELISA-based PSA tests.