BDNF Expression in Larval and Adult Zebrafish Brain: Distribution and Cell Identification.
BDNF Expression in Larval and Adult Zebrafish Brain: Distribution and Cell Identification.
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DOI：
10.1371/journal.pone.0158057
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Pellegrini E
中科院分区：
文献类型：
--
作者：
Cacialli P;Gueguen MM;Coumailleau P;D'Angelo L;Kah O;Lucini C;Pellegrini E
脑源性神经营养因子（BDNF）是神经营养因子家族的一员，在哺乳动物中枢神经系统的许多重要功能中发挥着重要作用。BDNF在神经发生、神经元成熟和/或突触可塑性中起重要作用，并参与认知功能，如学习和记忆。尽管有大量关于哺乳动物的文献，但对其他动物模型大脑中BDNF的研究却很少。斑马鱼是一种硬骨鱼，因发育遗传学研究而广为人知，并正在成为转化神经科学研究的模型。此外，它的大脑显示出许多成年神经发生的部位，在创伤性损伤后具有更高的再生特性。为了进一步了解脊椎动物脑模型中的神经营养因子，我们决定确定bdnf mRNA在斑马鱼幼体和成体脑中的分布，并通过双染色研究表征表达bdnf mRNA的细胞的表型。结果表明，bdnf基因在7日龄斑马鱼脑中广泛表达，在成熟的雌、雄斑马鱼全脑中也有表达。在成人中，bdnf mRNAs主要分布于端脑背侧、视前区、背侧丘脑、后结节、下丘脑、联脑、视顶盖和延髓。结合免疫组化和原位杂交，我们发现bdnf基因从来没有表达的放射状胶质细胞或增殖细胞。相比之下，bdnf转录本在所有研究的大脑区域的神经元表型细胞中表达。我们的研究结果提供了第一个证明，斑马鱼的大脑在神经元中表达BDNF mRNA，并开辟了新的研究领域，在正常和大脑修复的情况下，BDNF因子在大脑机制中的作用。
Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, has emerged as an active mediator in many essential functions in the central nervous system of mammals. BDNF plays significant roles in neurogenesis, neuronal maturation and/or synaptic plasticity and is involved in cognitive functions such as learning and memory. Despite the vast literature present in mammals, studies devoted to BDNF in the brain of other animal models are scarse. Zebrafish is a teleost fish widely known for developmental genetic studies and is emerging as model for translational neuroscience research. In addition, its brain shows many sites of adult neurogenesis allowing higher regenerative properties after traumatic injuries. To add further knowledge on neurotrophic factors in vertebrate brain models, we decided to determine the distribution of bdnf mRNAs in the larval and adult zebrafish brain and to characterize the phenotype of cells expressing bdnf mRNAs by means of double staining studies. Our results showed that bdnf mRNAs were widely expressed in the brain of 7 days old larvae and throughout the whole brain of mature female and male zebrafish. In adults, bdnf mRNAs were mainly observed in the dorsal telencephalon, preoptic area, dorsal thalamus, posterior tuberculum, hypothalamus, synencephalon, optic tectum and medulla oblongata. By combining immunohistochemistry with in situ hybridization, we showed that bdnf mRNAs were never expressed by radial glial cells or proliferating cells. By contrast, bdnf transcripts were expressed in cells with neuronal phenotype in all brain regions investigated. Our results provide the first demonstration that the brain of zebrafish expresses bdnf mRNAs in neurons and open new fields of research on the role of the BDNF factor in brain mechanisms in normal and brain repairs situations.