Uptake and conversion of d-amino acids in Arabidopsis thaliana
Uptake and conversion of d-amino acids in Arabidopsis thaliana
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DOI：
10.1007/s00726-010-0674-4
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发表时间：
2011-02-01
期刊：
影响因子：
3.5
通讯作者：
Thurow, Kerstin
中科院分区：
文献类型：
--
作者：
Goerdes, Dirk;Kolukisaoglu, Uener;Thurow, Kerstin
蛋白质氨基酸的d-对映体在细菌、真菌和动物中发挥重要作用。仅在植物界，d-氨基酸（d-AAs）的代谢和作用仍然不清楚，尽管植物必须科普来自根际微生物腐烂的大量这些化合物。为了填补这一空白的知识，我们测试了d-AAs对植物生长的抑制作用，并建立了一种方法来定量16的19个蛋白质氨基酸和它们的d-对映体在植物组织提取物。因此，在提取物中的氨基酸与Marveland的试剂衍生化和分离的HPLC-MS。我们使用两个生态型（Col-0和C24）和突变体（lht 1）的模式植物拟南芥，以确定外源应用的d-AA的影响和命运。所有这些都被发现在应用程序后的植物提取物中的高浓度，甚至在lht 1，这表明额外的转运促进进口的d-AA。添加特定氨基酸（d-Trp、d-Phe、d-Met和d-His）导致相应L-氨基酸的积累。在几乎所有情况下，应用d-AA导致d-Ala和d-Glu的积累。所呈现的结果表明，土壤中的d-AA可以积极采取和代谢通过中央代谢途径。
The d-enantiomers of proteinogenic amino acids fulfill essential functions in bacteria, fungi and animals. Just in the plant kingdom, the metabolism and role of d-amino acids (d-AAs) still remains unclear, although plants have to cope with significant amounts of these compounds from microbial decay in the rhizosphere. To fill this gap of knowledge, we tested the inhibitory effects of d-AAs on plant growth and established a method to quantitate 16 out of 19 proteinogenic amino acids and their d-enantiomers in plant tissue extracts. Therefore, the amino acids in the extracts were derivatized with Marfey's reagent and separated by HPLC-MS. We used two ecotypes (Col-0 and C24) and a mutant (lht1) of the model plant Arabidopsis thaliana to determine the influence and fate of exogenously applied d-AAs. All of them were found in high concentrations in the plant extracts after application, even in lht1, which points to additional transporters facilitating the import of d-AAs. The addition of particular amino acids (d-Trp, d-Phe, d-Met and d-His) led to the accumulation of the corresponding l-amino acid. In almost all cases, the application of a d-AA resulted in the accumulation of d-Ala and d-Glu. The presented results indicate that soil borne d-AAs can actively be taken up and metabolized via central metabolic routes.