Thiazole orange: A useful probe for fluorescence sensing of G-quadruplex-ligand interactions
Thiazole orange: A useful probe for fluorescence sensing of G-quadruplex-ligand interactions
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DOI：
10.1080/15257770701548212
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发表时间：
2007-01-01
影响因子：
1.3
通讯作者：
Teulade-Fichou, M. -P
中科院分区：
文献类型：
--
作者：
Monchaud, D.;Allain, C.;Teulade-Fichou, M. -P
进行荧光滴定以深入了解控制噻唑橙子（TO）和G-四链体-DNA（G4-DNA）缔合的参数。使用含环和缺乏环的四链体证明了环的化学计量的协会和荧光增强的TO的关键影响。我们随后试图从这种灵敏度中获益，通过最近报道的G4-FID测定来评估G4-DNA阳离子环境对配体结合的影响。
Fluorimetric titrations were performed to gain insight into parameters that govern the association of thiazole orange (TO) and G-quadruplex-DNA (G4-DNA). Use of loop-containing and loop-lacking quadruplexes evidenced the critical influence of the loops on the stoichiometry of the association and on the fluorescence exaltation of TO. We subsequently tried to benefit from this sensitivity to evaluate the influence of G4-DNA cationic environment on ligand binding via a recently reported G4-FID assay.