Enhancing enzymatic hydrolysis of corn cob, corn stover and sorghum stalk by dilute aqueous ammonia combined with ultrasonic pretreatment
Enhancing enzymatic hydrolysis of corn cob, corn stover and sorghum stalk by dilute aqueous ammonia combined with ultrasonic pretreatment
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DOI：
10.1016/j.indcrop.2017.08.038
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发表时间：
2017-12
影响因子：
5.9
通讯作者：
Qin‐Qin Xu;Meng-Jiao Zhao;Zhengfa Yu;Jianzhong Yin;Guo-min Li;Meng-Yuan Zhen;Qiao-Zhi Zhang
中科院分区：
文献类型：
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作者：
Qin‐Qin Xu;Meng-Jiao Zhao;Zhengfa Yu;Jianzhong Yin;Guo-min Li;Meng-Yuan Zhen;Qiao-Zhi Zhang
研究了在温和操作条件下超声辅助稀氨水预处理对玉米芯、玉米秸秆和高粱秆的强化酶解作用。研究了预处理温度（40–70 °C）、时间（1–4 h）和氨水浓度（1.0–4.0 wt%）对酶解糖产量（EH 糖产量）的影响。之后，分别对三种材料采用超声波、氨水和超声波联合氨水预处理进行了一系列对比实验。此外，还对预处理前后的样品进行了X射线衍射（XRD）和扫描电子显微镜（SEM），以研究生物质微观结构的变化。最后采用van Soest法对氨水或超声辅助氨水预处理后的原料和样品的主要成分进行分析，表明预处理的脱木素效果。研究发现，稀氨水结合超声波预处理可以提高生物质中纤维素的可及性，提高酶解糖得率。联合预处理后，玉米芯、玉米秸秆和高粱秆的最大EH糖得率分别为80.6%、66.2%和56.9%。
Ultrasound-assisted dilute aqueous ammonia pretreatment under mild operating conditions has been investigated for intensification of enzyme hydrolysis for corn cob, corn stover and sorghum stalk. The effect of the pretreatment temperature (40–70 °C), time (1–4 h) and aqueous ammonia concentration (1.0–4.0 wt%) on the enzymatic hydrolysis sugar yield (EH sugar yield) were investigated. After that, a series of comparative experiments were conducted using ultrasonic, aqueous ammonia and ultrasonic combined with aqueous ammonia pretreatments for the three materials respectively. In addition, X-ray diffraction (XRD) and Scanning Electron Microscope (SEM) of the samples before and after pretreatment were performed in order to investigate the change of the microscopic structures of the biomass. Finally, the main components of the raw materials and the samples after pretreatment by aqueous ammonia or ultrasound-assisted aqueous ammonia were analyzed by van Soest method, indicating the delignification by the pretreatment. It was found that dilute aqueous ammonia combined with ultrasonic pretreatment can improve the accessibility of cellulose in the biomass and improve the enzymatic hydrolysis sugar yield. The largest EH sugar yield was 80.6%, 66.2% and 56.9% for corn cob, corn stover and sorghum stalk, respectively, after the combined pretreatment.