Ethanol perturbs lipid organization in models of stratum corneum membranes: An investigation combining differential scanning calorimetry, infrared and 2H NMR spectroscopy
Ethanol perturbs lipid organization in models of stratum corneum membranes: An investigation combining differential scanning calorimetry, infrared and 2H NMR spectroscopy
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DOI：
10.1016/j.bbamem.2012.02.013
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发表时间：
2012-05-01
影响因子：
3.4
通讯作者：
Thewalt, Jenifer
中科院分区：
文献类型：
--
作者：
Kwak, Sungjong;Brief, Elana;Thewalt, Jenifer
乙醇用于各种局部产品。已知通过改变角质层（SC）细胞间膜形成有效屏障的能力来增强皮肤的渗透性。此外，乙醇和其他醇类是目前用于洗手的抗菌凝胶的关键成分。使用红外和氘核磁共振光谱以及量热法，我们已经研究了乙醇对模型膜组成的脂质代表三个类的SC脂质，N-棕榈酰鞘氨醇（神经酰胺），棕榈酸和胆固醇的等摩尔混合物的影响。发现乙醇以剂量依赖性方式影响膜，在低浓度下破坏包装并增加脂质运动，并在中等浓度下选择性地提取脂质。(C)2012爱思唯尔有限公司版权所有。
Ethanol is used in a variety of topical products. It is known to enhance the permeability of the skin by altering the ability of the stratum corneum (SC) intercellular membranes to form an effective barrier. In addition, ethanol and other alcohols are key components of antiseptic gels currently used for hand wash. Using infrared and deuterium NMR spectroscopy as well as calorimetry, we have investigated the effect of ethanol on a model membrane composed of lipids representing the three classes of SC lipids, an equimolar mixture of N-palmitoylsphingosine (ceramide), palmitic acid and cholesterol. Ethanol is found to influence the membrane in a dose dependent manner, disrupting packing and increasing lipid motion at low concentrations and selectively extracting lipids at moderate concentrations. (C) 2012 Elsevier B.V. All rights reserved.