DEAD-box proteins from Escherichia coli exhibit multiple ATP-independent activities.
DEAD-box proteins from Escherichia coli exhibit multiple ATP-independent activities.
复制标题
来自大肠杆菌的 DEAD-box 蛋白表现出多种不依赖于 ATP 的活性。
DOI：
10.1128/jb.01488-10
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发表时间：
2011
影响因子：
3.2
通讯作者：
Jain,Chaitanya
中科院分区：
文献类型：
--
作者：
Zhao,Xinliang;Jain,Chaitanya
死亡盒蛋白（Dead-box proteins，DBP）是一类广泛存在的ATP依赖性RNA解旋酶，在RNA双链体解旋中起着关键作用。近年来，还发现某些DBP表现出不需要ATP的活动。为了更好地了解原核生物的RNA代谢，我们研究了大肠杆菌DBPs是否具有任何ATP非依赖性活性。我们证明了四个E.在这项研究中测试的coliDBPs可以加速cRNA分子的缔合，可以刺激链置换，并且可以在不利用ATP的情况下作为RNA伴侣发挥作用。据我们所知，这些原核DBPs构成了具有这三种活性的蛋白质的第一个例子。这些辅助功能的鉴定表明E.大肠杆菌DBP是多功能因子，除了其典型的RNA解旋酶活性外，还具有显著的RNA重塑活性，因此可能参与比以前认识到的更多种类的细胞过程。
DEAD-box proteins (DBPs) are a widespread class of ATP-dependent RNA helicases that play a key role in unwinding RNA duplexes. In recent years, certain DBPs have also been found to exhibit activities that do not require ATP. To gain a better understanding of prokaryotic RNA metabolism, we investigated whether Escherichia coli DBPs harbor any ATP-independent activities. We show that each of the four E. coli DBPs tested in this study can accelerate the association of cRNA molecules, can stimulate strand displacement, and can function as an RNA chaperone without utilizing ATP. To the best of our knowledge, these prokaryotic DBPs constitute the first examples of proteins that harbor each of these three activities. The identification of these auxiliary functions indicates that the E. coli DBPs are versatile factors that possess significant RNA remodeling activity in addition to their canonical RNA helicase activity and might therefore participate in a greater variety of cellular processes than has been previously appreciated.