Biomarkers of oxidative stress study:: Are plasma antioxidants markers of CC14 poisoning?
Biomarkers of oxidative stress study:: Are plasma antioxidants markers of CC14 poisoning?
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DOI：
10.1016/s0891-5849(00)00198-2
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发表时间：
2000-03-15
影响因子：
7.4
通讯作者：
Barrett, JC
中科院分区：
文献类型：
--
作者：
Kadiiska, MB;Gladen, BC;Barrett, JC
大鼠血浆中的抗氧化剂是作为一项全面的多实验室验证研究的一部分进行测量的，该研究旨在寻找氧化应激的非侵入性生物标志物。在这项初步研究中，研究了四氯化碳中毒的动物模型。研究了CCl 4对α-生育酚、辅酶Q（CoQ）、抗坏血酸、谷胱甘肽（GSH和GSSG）、尿酸和总抗氧化能力的血浆水平的时间（2、7和16 h）和剂量（120和1200 mg/kg，腹腔注射）依赖性影响，以确定CCl 4的氧化作用是否会导致血浆中抗氧化剂的损失。α-生育酚和辅酶Q的浓度在四氯化碳治疗的大鼠下降。由于胆固醇和甘油三酯的伴随降低，不可能将α-生育酚的氧化和辅酶Q的损失与由于肝损伤引起的全身脂质变化分离。在最早的时间点，抗坏血酸水平较高，GSH与GSSG的比值普遍下降，尿酸保持不变。总抗氧化能力没有显着的变化，除了16小时后，高剂量，当它增加。这些结果表明，肝功能障碍和肝细胞破裂引起的血浆变化以及血浆脂质降低不允许对结果进行明确解释，并妨碍检测血浆抗氧化状态的任何潜在变化。(C)2000 Elsevier Science Inc.
Antioxidants in the blood plasma of rats were measured as part of a comprehensive, multilaboratory validation study searching for noninvasive biomarkers of oxidative stress. For this initial study an animal model of CCl4 poisoning was studied. The time (2, 7, and 16 h) and dose (120 and 1200 mg/kg, intraperitoneally)-dependent effects of CCl4 on plasma levels of alpha-tocopherol, coenzyme Q (CoQ), ascorbic acid, glutathione (GSH and GSSG), uric acid, and total antioxidant capacity were investigated to determine whether the oxidative effects of CCl4 would result in losses of antioxidants from plasma. Concentrations of alpha-tocapherol and CoQ were decreased in CCl4-treated rats. Because of concomitant decreases in cholesterol and triglycerides, it was impossible to dissociate oxidation of alpha-tocopherol and the loss of CoQ from generalized lipid changes, due to liver damage. Ascorbic acid levels were higher with treatment at the earliest time point; the ratio of GSH to GSSG generally declined, and uric acid remained unchanged. Total antioxidant capacity showed no significant change except for 16 h after the high dose, when it was increased. These results suggest that plasma changes caused by liver malfunction and rupture of liver cells together with a decrease in plasma lipids do not permit an unambiguous interpretation of the results and impede detection of any potential changes in the antioxidant status of the plasma. (C) 2000 Elsevier Science Inc.