The expression of cyclooxygenase-2 (COX-2) in amnion and decidua following spontaneous labor
The expression of cyclooxygenase-2 (COX-2) in amnion and decidua following spontaneous labor
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DOI：
10.1016/s0090-6980(96)00088-3
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发表时间：
1996-10-01
期刊：
影响因子：
--
通讯作者：
OBrien, WF
中科院分区：
文献类型：
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作者：
Fuentes, A;Spaziani, EP;OBrien, WF
目的：在足月和早产期间前列腺素的产生急剧增加。这种产物的来源被认为是胎膜和母体蜕膜。负责将花生四烯酸转化为胡萝卜素和相关内过氧化物的酶被称为前列腺素合酶或环氧合酶（考克斯）。这种酶的诱导型考克斯-2在几种组织中均有报道。本研究的目的是探讨可能的作用，考克斯-2在劳动力比较考克斯-2含量在羊膜和蜕膜从劳动和non-laboring patients.Study Design：胎膜从7个正常劳动和10个择期剖宫产在足月分娩后立即收集。两组之间的产妇年龄和体重相似。鉴定羊膜和蜕膜，在无菌盐水中洗涤，在液氮中冷冻并储存在-70 ℃下。使用纯化的考克斯-2抗体，使用蛋白质印迹分析测定考克斯-2表达。使用扫描光密度计对条带进行定量。结果表示为平均值+/- S.D.。结果：与未分娩组相比，分娩妇女羊膜中考克斯-2的浓度显示出两倍的增加（240.0 +/- 17.6 vs.120.7 +/- 5.1）（p0.05）。结论：我们评估了考克斯-2在正常分娩中的作用。我们的研究表明，考克斯-2显着诱导羊膜后自然分娩。提示羊膜考克斯-2的诱导表达可能参与了人类的分娩过程。
Objective: Prostaglandins production rises dramatically during term and preterm labor. The source of this production is thought to be the fetal membranes and maternal decidua. The enzyme responsible for the conversion of arachidonic acid to the prostaglandins and related endoperoxides is variously known as prostaglandin synthase or cyclooxygenase (COX). An inducible form of this enzyme, COX-2, has been described in several tissues. The purpose of this study was to investigate a possible role for COX-2 in labor by comparing the COX-2 content in amnion and decidua from laboring and non-laboring patients.Study Design: Fetal membranes from seven normal labor and ten elective cesarean section at term were collected immediately following delivery. The maternal age and gravity were similar between the groups. The amnion and decidua were identified, washed in sterile saline, frozen in liquid nitrogen and stored in -70 degrees C. COX-2 expression was determined using Western Blot analysis with a purified COX-2 antibody. A scanning densitometer was used to quantify the bands. Results were expressed as mean +/- S.D. ng/50 mu g protein.Results: The concentration of COX-2 in amnion of laboring women showed a twofold increase (240.0 +/- 17.6 vs. 120.7 +/- 5.1) compared to the non-labored group (p0.05).Conclusion: We evaluated the role of COX-2 in normal labor. Our study demonstrates that COX-2 is significantly induced in the amnion following spontaneous labor. These findings suggest that the induction of amnion COX-2 may be involved in the process of human labor.