Local regulation of auxin transport in root-apex transition zone mediates aluminium-induced Arabidopsis root-growth inhibition
Local regulation of auxin transport in root-apex transition zone mediates aluminium-induced Arabidopsis root-growth inhibition
复制标题
根尖过渡区生长素运输的局部调节介导铝诱导的拟南芥根生长抑制
DOI：
10.1111/tpj.15424
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发表时间：
2021
期刊：
影响因子：
7.2
通讯作者：
Yang Zhong-Bao
中科院分区：
文献类型：
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作者：
Li Cuiling;Liu Guangchao;Geng Xiaoyu;He Chunmei;Quan Taiyong;Hayashi Ken-Ichiro;De Smet Ive;Robert Helene S.;Ding Zhaojun;Yang Zhong-Bao
铝胁迫是酸性土壤上世界性作物生产的主要限制因素。在拟南芥中，根尖过渡区(TZ)中依赖于TAA1的局部生长素生物合成是铝诱导根生长抑制的关键，而铝调控生长素在TZ中积累的机制尚不完全清楚。本研究探讨了生长素运输在铝诱导的TZ生长素局部积累和根生长抑制中的作用。结果表明，PIN形成的PIN蛋白如PIN1、PIN3、PIN4和PIN7以及AUX1/LAX蛋白如AUX1、LAX1和LAX2均在根尖对铝胁迫的响应中异位上调，并协调调节生长素在根尖的积累和根的生长抑制。铝胁迫下TZ中Pin1的异位上调受乙烯和生长素共同调控，生长素信号作用于乙烯下游。铝诱导的Pin1上调和生长素在根尖TZ的积累也受到类Calossin蛋白BIG的调节。综上所述，我们的结果揭示了铝胁迫是如何通过局部调节生长素运输来诱导生长素在TZ的局部积累和抑制根的生长的。
Aluminium (Al) stress is a major limiting factor for worldwide crop production in acid soils. InArabidopsis thaliana, the TAA1‐dependent local auxin biosynthesis in the root‐apex transition zone (TZ), the major perception site for Al toxicity, is crucial for the Al‐induced root‐growth inhibition, while the mechanism underlying Al‐regulated auxin accumulation in the TZ is not fully understood. In the present study, the role of auxin transport in Al‐induced local auxin accumulation in the TZ and root‐growth inhibition was investigated. Our results showed that PIN‐FORMED (PIN) proteins such as PIN1, PIN3, PIN4 and PIN7 and AUX1/LAX proteins such as AUX1, LAX1 and LAX2 were all ectopically up‐regulated in the root‐apex TZ in response to Al stress and coordinately regulated local auxin accumulation in the TZ and root‐growth inhibition. The ectopic up‐regulation of PIN1 in the TZ under Al stress was regulated by both ethylene and auxin, with auxin signalling acting downstream of ethylene. Al‐induced PIN1 up‐regulation and auxin accumulation in the root‐apex TZ was also regulated by the calossin‐like protein BIG. Together, our results provide insight into how Al stress induces local auxin accumulation in the TZ and root‐growth inhibition through the local regulation of auxin transport.