TRANSACTIVATION OF ANTHOCYANIN BIOSYNTHETIC GENES FOLLOWING TRANSFER OF B-REGULATORY GENES INTO MAIZE TISSUES
TRANSACTIVATION OF ANTHOCYANIN BIOSYNTHETIC GENES FOLLOWING TRANSFER OF B-REGULATORY GENES INTO MAIZE TISSUES
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DOI：
10.1002/j.1460-2075.1990.tb07431.x
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发表时间：
1990-08-01
期刊：
影响因子：
11.4
通讯作者：
CHANDLER, VL
中科院分区：
文献类型：
--
作者：
GOFF, SA;KLEIN, TM;CHANDLER, VL
调节玉米花青素生物合成途径的 C1、B 和 R 基因编码具有转录激活因子相似性的组织特异性调节蛋白。 C1 和 R 调节基因通常负责种子组织的色素沉着，B 的 B-Peru 等位基因（但不是 B-I 等位基因）可以替代种子中的 R 功能。在这项研究中，B 调控基因家族的成员通过高速微弹传递到完整的玉米组织中。在无色糊粉或胚胎中，引入B-Peru基因组克隆或B-Peru或B-I表达的cDNA导致产生花青素的细胞。当与表达的 B-Peru 或 B-I cDNA 共同引入时，由 Bronze1 花青素结构基因启动子产生的荧光素酶被诱导 100 倍。这种定量反式激活测定表明，这两个 B 等位基因编码的蛋白质同样能够反式激活 Bronze1 启动子。使用胚胎发生愈伤组织细胞获得了类似的结果。这些观察结果表明，B 和 R 基因的各种等位基因控制的组织特异性花青素合成的一个主要贡献是功能相似蛋白质的差异表达。
The C1, B and R genes regulating the maize anthocyanin biosynthetic pathway encode tissue-specific regulatory proteins with similarities of transcriptional activators. The C1 and R regulatory genes are usually responsible for pigmentation of seed tissues, and the B-Peru allele of B, but not the B-I allele, can substitute for R function in the seed. In this study, members of the B family of regulatory genes were delivered in intact maize tissues by high velocity microprojectiles. In colorless r aleurones or embryos, the introduction of the B-Peru genomic clone or the expressed cDNAs of B-Peru or B-I resulted in anthocyanin-producing cells. Luciferase produced from the Bronze1 anthocyanin structural gene promoter was induced 100-fold when co-introduced with the expressed B-Peru or B-I cDNAs. This quantitative transactivation assay demonstrates that the proteins encoded by these two B alleles are equally able to transactivate the Bronze1 promoter. Analogous results were obtained using embryogenic callus cells. These observations suggest that one major contribution towards tissue-specific anthocyanin synthesis controlled by the various alleles of the B and R genes is the differential expression of functionally similar proteins.