Ca2+ signaling via the neuronal calcium sensor-1 regulates associative learning and memory in C-elegans
Ca2+ signaling via the neuronal calcium sensor-1 regulates associative learning and memory in C-elegans
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DOI：
10.1016/s0896-6273(01)00276-8
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发表时间：
2001-04-01
期刊：
影响因子：
16.2
通讯作者：
Nef, P
中科院分区：
文献类型：
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作者：
Gomez, M;De Castro, E;Nef, P
在径向温度梯度上，线虫在用食物调节后向其培养温度迁移并沿着这条等温线移动。这种依赖于经验的行为称为等温跟踪 (IT)。在这里，我们证明神经元特异性钙传感器 1 (NCS-1) 对于优化 IT 至关重要。 ncs-1 基因敲除动物的 IT 行为表现出重大缺陷，尽管它们的趋化、运动和热回避行为是正常的。通过将野生型 NCS-1 重新引入 AIY 中间神经元（趋热网络的关键组成部分），可以挽救敲除表型。无法结合钙的 NCS-1 功能丧失形式不会恢复 IT，而 NCS-1 过度表达可增强 IT 性能水平，加速学习（更快的获取），并产生消退较慢的记忆。因此，通过 NCS-1 的适当钙信号传导定义了一条对于联想学习和记忆至关重要的新途径。
On a radial temperature gradient, C. elegans worms migrate, after conditioning with food, toward their cultivation temperature and move along this isotherm. This experience-dependent behavior is called isothermal tracking (IT). Here we show that the neuron-specific calcium sensor-1 (NCS-1) is essential for optimal IT. ncs-1 knockout animals show major defects in IT behavior, although their chemotactic, locomotor, and thermal avoidance behaviors are normal. The knockout phenotype can be rescued by reintroducing wildtype NCS-1 into the AIY interneuron, a key component of the thermotaxis network. A loss-of-function form of NCS-1 incapable of binding calcium does not restore IT, whereas NCS-1 overexpression enhances IT performance levels, accelerates learning (faster acquisition), and produces a memory with slower extinction. Thus, proper calcium signaling via NCS-1 defines a novel pathway essential for associative learning and memory.