In vivo transplantation of bone marrow mesenchymal stem cells accelerates repair of injured gastric mucosa in rats
In vivo transplantation of bone marrow mesenchymal stem cells accelerates repair of injured gastric mucosa in rats
复制标题
骨髓间充质干细胞体内移植加速大鼠受损胃粘膜修复
DOI：
10.3760/cma.j.issn.0366-6999.2012.06.036
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发表时间：
2012-03-20
影响因子：
6.1
通讯作者：
Wu Ben-yan
中科院分区：
文献类型：
--
作者：
Chang Qing;Yan Li;Wu Ben-yan
背景成体干细胞为损伤组织的治疗提供了一种有前途的替代方案。目的探讨骨髓间充质干细胞（BMMSCs）体内移植对大鼠胃黏膜损伤的治疗作用。用荧光细胞连接剂5，6-羧基荧光素二乙酸琥珀酰亚胺酯（CFDA SE）标记的雄性大鼠BMMSCs通过尾静脉注射移植到雌性大鼠中。HE染色观察溃疡愈合过程。结果骨髓间充质干细胞移植后48 h和72 h，在损伤胃黏膜中可见散在分布的CFDA SE标记细胞，而对照组胃黏膜中未见CFDA SE标记细胞。在BMMSCs移植后72小时，BMMSCs移植组的平均溃疡指数为12.67 +/- 2.16，对照组为17.33 +/- 1.97（P
Background Adult stem cells provide a promising alternative for the treatment of injured tissues. We aimed to investigate the effect of in vivo transplantation of bone marrow mesenchymal stem cells (BMMSCs) on injured gastric mucosa in rats.Methods The gastric ulcer in rats was induced by indomethacin. BMMSCs from male rats, labeled with the fluorescent cell linker 5,6-carboxyfluorescein diacetate succinimidyl ester (CFDA SE), were transplanted into the female rats via tail vein injection. The healing process of gastric ulcers was monitored by HE staining. The protein levels of vascular endothelial growth factor (VEGF) and the epidermal growth factor receptor (EGFR) in the injured gastric mucosa were determined by immunohistochemistry.Results At 48 and 72 hours after BMMSCs transplantation, the CFDA SE labeled cells were found scattered in the injured gastric mucosa, but not in the gastric mucosa of control rats. At 72 hours after BMMSCs transplantation, the mean ulcer index was 12.67 +/- 2.16 in the BMMSCs transplanted group and 17.33 +/- 1.97 in vehicle-treated controls (P