Expression and clinical significance of A-kinase anchor protein 4 in lung adenocarcinoma tissue.
Expression and clinical significance of A-kinase anchor protein 4 in lung adenocarcinoma tissue.
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DOI：
10.1111/1759-7714.12324
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发表时间：
2016-04-26
期刊：
影响因子：
2.9
通讯作者：
Xu P
中科院分区：
文献类型：
--
作者：
Li HM;Guo K;Yu XY;Yu Z;Xu P
A-激酶锚蛋白（AKAP）是一个不断增长的支架蛋白家族，通过控制细胞内信号参与肿瘤的发生、增殖和转移。本研究分析了AKAP 4在肺腺癌患者及癌旁非癌组织中的表达及意义。采用逆转录聚合酶链反应和蛋白质印迹法检测108例肺腺癌及癌旁组织中AKAP 4 mRNA和蛋白的表达水平。 AKAP 4 mRNA和蛋白在肺腺癌组织中表达，而在癌旁组织中不表达。AKAP 4 mRNA和蛋白的表达与淋巴结转移密切相关（P < 0.05），而与临床分期、分化程度、性别、年龄、吸烟无关（P > 0.05）。AKAP 4表达对总生存率有不利影响（P < 0.05）。AKAP 4在肺腺癌组织中呈高表达，可能与肺腺癌的淋巴结转移密切相关。AKAP 4可能是一种新的肺腺癌分子标志物和预后不良的预测因子。
The A‐kinase anchor proteins (AKAP) are a growing family of scaffolding proteins involved in the occurrence, proliferation, and metastasis of tumors by controlling intracellular signals. In this study, the expression and significance of AKAP4 were analyzed in patients with lung adenocarcinoma and adjacent non‐cancerous tissues. Using reverse transcriptase‐polymerase chain reaction and Western blot, AKAP4 messenger ribonucleic acid (mRNA) and protein expression levels were measured in 108 cases of lung adenocarcinoma and adjacent non‐cancerous tissues. AKAP4 mRNA and protein were expressed in lung adenocarcinoma tissues, but not in adjacent non‐cancerous tissues. The expression of AKAP4 mRNA and protein was closely associated with lymphatic metastasis (P < 0.05), but had no relationship with stage, differentiation degree, gender, age or smoking (P > 0.05). AKAP4 expression had an adverse effect on the overall survival rate (P < 0.05). The expression of AKAP4 was high in lung adenocarcinoma tissue, which may be closely related to the lymphatic metastasis of lung adenocarcinoma. AKAP4 may be a novel lung adenocarcinoma molecule marker and a predictor of poor prognosis.