Clonal analysis of leaf development in cotton
Clonal analysis of leaf development in cotton
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DOI：
10.2307/2446322
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发表时间：
1998-03-01
影响因子：
3
通讯作者：
Poethig, RS
中科院分区：
文献类型：
--
作者：
Dolan, L;Poethig, RS
用克隆分析方法描述了美国皮马棉(Gossypium Barbadense)叶片发育的细胞学参数。叶片分化前诱导的扇形(无性系)表明，叶原基由茎分生组织侧翼上类似100个细胞形成。对叶片展开期间扇形频率的分析表明，细胞分裂速率在整个叶片长度上相当均匀，但叶片边缘的细胞分裂速率低于间叶区域。边缘扇区的形状表明，该区域的细胞分裂方向(由新细胞壁的方向定义)更多地平行于边缘而不是垂直于边缘，尽管极化程度沿边缘的长度变化。在发育后期，细胞分裂的持续时间沿叶片的长度有一个轻微的梯度，在两个细胞周期中，细胞分裂从叶片顶端到底部逐渐停止。因此，棉花的细胞分裂参数与烟草中描述的参数相似，但显著的例外是叶缘细胞的行为。
Clonal analysis has been used to describe the cellular parameters of leaf development in American Pima cotton (Gossypium barbadense). Sectors (clones) induced before leaf initiation indicate that the leaf primordium arises from similar to 100 cells on the flank of the shoot meristem. An analysis of sector frequency during the period of leaf expansion suggests that the rate of cell division is fairly uniform throughout the length of the leaf, but is lower at the margin of the lamina than in intercalary regions. The shapes of marginal sectors indicate that the orientation of cell division (as defined by the orientation of the new cell wall) in this region is more often parallel to the margin than perpendicular to it, although the degree of polarization varies along the length of the margin. There is a slight gradient in the duration of cell division along the length of of the lamina late in development, with cell division ceasing progressively from the lamina tip to the base over two cell cycles. The parameters of cell division in cotton are therefore similar to those described for tobacco with the notable exception of the behavior of cells at the leaf margin.