Tetracycline fiber therapy monitored by DNA probe and cultural methods.
Tetracycline fiber therapy monitored by DNA probe and cultural methods.
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通过 DNA 探针和培养方法监测四环素纤维疗法。
DOI：
10.1111/j.1600-0765.1991.tb01735.x
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发表时间：
1991
影响因子：
3.5
通讯作者：
Goodson,JM
中科院分区：
文献类型：
--
作者：
Maiden,MF;Tanner,A;McArdle,S;Najpauer,K;Goodson,JM
在一项评估局部四环素纤维疗法的研究中，比较了寡核苷酸 DNA 探针和选择性培养方法监测 6 种假定牙周病原体的能力。从 20 名受试者每人的 4 个部位采集龈下菌斑样本。在治疗前后从分配给以下测试组的部位采集样本：四环素（TC）纤维、刮治和根面平整、对照纤维和未经处理的纤维。每个样本均通过 DNA 探针和培养方法进行分析。在非选择性血琼脂上计数厌氧菌总数、牙龈卟啉单胞菌（拟杆菌）和中间普雷沃氏菌（中间拟杆菌）。在选择性培养基上分离放线放线杆菌、腐蚀艾肯氏菌、具核梭杆菌和直肠沃利氏菌。 TC 纤维治疗和缩放将可培养总计数从初始值 1 × 107 减少到治疗后的约 2 × 105。未处理部位和有对照纤维部位的总计数与基线相比没有变化。伴放线菌和 E.培养法更频繁地检测到腐蚀物；其他受监测物种通过 DNA 探针检测的频率高于通过培养方法检测的频率。方法之间的一致性为：A 为 77.2%。伴放线菌； 72.2% 为 P。中间媒体； 75.6% 为E。腐蚀； F 为 39.4%。有核细胞； 35.6% 为 P。牙龈; W 为 68.9%。直肠。所使用的选择性培养方法的局限性可能导致了 P 的差异。牙龈和F.有核细胞。 DNA 探针和培养方法表明，TC 纤维治疗和刮治后对受监测物种的抑制水平相当。两种方法似乎都没有显着改变对照纤维和未处理部位的微生物群。
Oligonucleotide DNA probe and selective cultural methods were compared in their ability to monitor 6 putative periodontal pathogens in a study evaluating local tetracycline fiber therapy. Subgingival plaque was sampled from 4 sites in each of 20 subjects. Samples were taken before and after therapy from sites assigned to the following test groups: tetracycline (TC) fiber, scaling and root planing, control fiber, and untreated. Each sample was analyzed by both DNA probe and cultural methods. Total anaerobic cultivable counts,Porphyromonas (Bacteroides) gingivalisandPrevotella intermedia (Bacteroides intermedius)were enumerated on nonselective blood agar.Actinobacillus actinomycetemcomitans, Eikenella corrodens, Fusobacterium nucleatumandWolinella rectawere isolated on selective media. TC fiber therapy and scaling reduced total cultivable counts from an initial value of 1 × 107to approximately 2 × 105following therapy. Total counts at untreated sites and at sites with control fibers did not change from baseline.A. actinomycetemcomitansandE. corrodenswere detected more frequently by the cultural method; the other monitored species were detected more frequently by DNA probes than by the cultural methods. Agreements between methods were: 77.2% forA. actinomycetemcomitans; 72.2% forP. intermedia; 75.6% forE. corrodens; 39.4% forF. nucleatum; 35.6% forP. gingivalis; and 68.9% forW. recta. Limitations of the selective cultural methods used probably contributed to the discrepancies forP. gingivalisandF. nucleatum. DNA probe and cultural methods indicated comparable levels of suppression of the monitored species following TC fiber therapy and scaling. The microbiota of control fiber and untreated sites did not appear to be significantly altered by either method.