New type of glucose sensor based on enzymatic conversion of gel volume into liquid column length.
New type of glucose sensor based on enzymatic conversion of gel volume into liquid column length.
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DOI：
10.1021/bm034331q
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发表时间：
2004-03
影响因子：
6.2
通讯作者：
H. Suzuki;A. Kumagai;K. Ogawa;E. Kokufuta
中科院分区：
文献类型：
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作者：
H. Suzuki;A. Kumagai;K. Ogawa;E. Kokufuta
提出了一种将生化机械凝胶的体积变化转化为液柱长度变化的方法。我们的实验传感器装置包括一个小隔间，用于容纳凝胶，一个装有填充染料溶液的流动通道，以及一个用于分离凝胶和染料溶液的聚二甲基硅氧烷(PDMS)隔膜。以N-异丙基丙烯酰胺(NIPAAm)/丙烯酸(AA)共聚凝胶为传感元件，固定化葡萄糖氧化酶。研究发现，在PDMS隔膜的帮助下，固定化酶反应引起的凝胶体积的变化可以准确地转化为柱长的变化(Deltal)。通过使用圆柱形凝胶(直径约2，厚度约1 mm)，Deltal的时间曲线在0.2-50 mm的范围内随葡萄糖浓度的变化而变化；尤其重要的是，Deltal(Mm)与葡萄糖浓度(Mm)的半对数曲线可用作校准曲线。对于毫米级的葡萄糖溶液，1分钟就足以确定浓度，而对于微米级的浓度则需要10分钟。当测量时间控制在10min以内时，检测下限为200微米。响应受样品缓冲能力的影响，但这是通过减少样品体积来控制的。这些结果表明，本方法可用于葡萄糖浓度的测定。
A way to convert the volume change of a biochemo-mechanical gel into the change in liquid column length was developed. Our trial sensor device consisted of a small compartment for incorporating the gel, a flow channel with a filled dye solution, and a poly(dimethylsiloxane) (PDMS) diaphragm by which the gel and the dye solution were separated. A lightly cross-linked N-isopropylacrylamide (NIPAAm)/acrylic acid (AA) copolymer gel with immobilized glucose oxidase was used as a sensing element. It was found that a change in the gel volume caused by the immobilized enzyme reaction was accurately converted into a change of the column length (Deltal) with the help of the PDMS diaphragm. By use of a cylindrical gel (diameter approximately 2 and thickness approximately 1 mm), the time curve of Deltal varied depending upon glucose concentration over a range of 0.2-50 mM; in particular, it is of importance that semilogarithmic plots of Deltal (in mm) against glucose concentration (in mM) can be used as a calibration curve. For glucose solutions of mM order, 1 min was enough to determine the concentrations, whereas 10 min was required for concentrations of microM order. When the measurement time was limited within 10 min, the lower detection limit was 200 microM. The response was affected by buffering capacity of the samples, but this was controllable through reduction of the sample volume. These results indicate that the present way can be used for the determination of glucose concentration.