TectoRNA and 'kissing-loop' RNA: atomic force microscopy of self-assembling RNA structures
TectoRNA and 'kissing-loop' RNA: atomic force microscopy of self-assembling RNA structures
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DOI：
10.1111/j.1365-2818.2003.01276.x
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发表时间：
2003-12-01
影响因子：
2
通讯作者：
Jaeger, L
中科院分区：
文献类型：
--
作者：
Hansma, HG;Oroudjev, E;Jaeger, L
与 DNA 分子相比，原子力显微镜 (AFM) 对 RNA 分子的研究要少得多。在本文中，提出了两种不同的 RNA 分子的 AFM 成像，它们能够自组装成复杂的超分子结构。第一个是来自鼠白血病病毒 RNA 基因组的 230 nt RNA 片段的分子二聚体。单体 RNA 片段在 AFM 中显示为平均长宽比为 1.4 的细长结构，通过形成“接吻环”RNA 相互作用组装成细长结构的二聚体。第二种是由人工自组装 RNA 分子单元（称为 tectoRNA）形成的大型超分子纤维。 AFM 的纤维长度表明每根纤维有 50-70 个 tectoRNA 单位。提出了从 AFM 图像测量分子体积的一些方法和限制。
RNA molecules have been much less studied by atomic force microscopy (AFM) than have DNA molecules. In this paper, AFM imaging is presented for two different RNA molecules able to self-assemble into complex supramolecular architectures. The first one is a molecular dimer of a 230-nt RNA fragment coming from the RNA genome of a murine leukaemia virus. The monomeric RNA fragment, which appears by AFM as an elongated structure with a mean aspect ratio of 1.4, assembles into a dimer of elongated structures through the formation of a 'kissing-loop' RNA interaction. The second one is a large supramolecular fibre formed of artificial self-assembling RNA molecular units called tectoRNA. The fibre lengths by AFM suggest that there are 50-70 tectoRNA units per fibre. Some methods and limitations are presented for measuring molecular volumes from AFM images.