Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes
Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes
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妊娠血清对 B 淋巴细胞选择性表达的非 HL-A 同种异体抗原的识别
DOI：
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发表时间：
1975
影响因子：
15.3
通讯作者：
S. Fu
中科院分区：
文献类型：
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作者：
R. Winchester;P. Wernet;H. Kunkel;B. Dupont;C. Jersild;S. Fu
在妊娠血清中发现了一组同种异体抗体，它们与B淋巴细胞和单核细胞上的抗原发生反应，但在绝大多数未刺激的T细胞上检测不到。这种特异性将它们与HL-A抗体区分开来，HL-A抗体与这两种细胞类型都有反应。利用B细胞淋巴系和正常外周血T细胞相结合的间接荧光抗体分析很容易识别它们。不同的血清对11条淋巴系有不同的反应模式。来自不同个体的正常B细胞也观察到相似的分化模式，特别是在浓缩B细胞后。抗体对B细胞也有细胞毒作用，这一方法的结果与荧光法相似。获得的反应模式表明，这是一个与HL-A类似的非常不均匀的系统。对某些血清的特殊研究提供了证据，表明混合淋巴细胞反应系统中涉及淋巴细胞定义的决定因素。为方便起见，术语HL-B被用来表示这些抗原。
A group of alloantibodies are found in pregnancy sera which react with antigens present on B lymphocytes and monocytes but are not detectable on the vast majority of unstimulated T cells. This specificity distinguishes them from HL-A antibodies which react with both cell types. They were readily recognized through indirect fluorescent antibody analysis by employing the combination of B-cell lymphoid lines and normal peripheral blood T cells. Different sera gave a variety of patterns of reactivity with a panel of 11 lymphoid lines. Similar differential patterns were also observed with normal B cells from different individuals particularly after concentrating the B cells. The antibodies were also cytotoxic to B cells and this procedure gave parallel results to the fluorescence method. The pattern of reactions obtained indicated a very heterogeneous system similar to that for HL- A. Special study of certain of the sera provided evidence that the lymphocyte-defined determinants of the mixed lymphocyte reaction system were involved. For convenience the term HL-B has been employed for these antigens.