Correlation between circulating HIV-1 RNA and broad HIV-1 neutralizing antibody activity.
Correlation between circulating HIV-1 RNA and broad HIV-1 neutralizing antibody activity.
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DOI：
10.1097/qai.0b013e3182100c1b
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发表时间：
2011-05-01
期刊：
影响因子：
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通讯作者：
Redfield RR
中科院分区：
文献类型：
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作者：
Sajadi MM;Guan Y;DeVico AL;Seaman MS;Hossain M;Lewis GK;Redfield RR
检查 HIV-1 抗原负荷（血浆 RNA 拷贝数/ml）与广泛的 HIV-1 中和抗体活性之间的关系。来自 120 名 HIV-1 感染患者的血浆，包括 HIV-1 天然病毒抑制剂（类似于精英控制者），经过了针对 15 种 1 级/2 级 HIV-1 假病毒的中和测试。通过 IgG 和异源进化枝测试（来自进化枝 A、C 和 CRF02_AG 的 18 种假病毒）证实了广泛的 HIV-1 中和抗体活性。进行统计分析以确定与广泛的 HIV-1 中和抗体活性相关的因素。鉴定出 10 个人具有广泛的 HIV-1 中和抗体活性。这些人的 CD4 计数中位数为 589 个细胞/ul（范围 202-927），HIV-1 RNA 拷贝数为 1,611 个/ml（范围 110-8,964），自 HIV 诊断以来已有 13 年（范围 1-22）。 HIV-1 RNA 介于 100 至 10,000 拷贝/ml 的人群与 <100 或 >10,000 拷贝/ml 的人群相比，广泛中和抗体的存在之间存在显着相关性（分别为 p=0.0003 和 0.0245）。与 <100 或 >10,000 拷贝/ml 组相比，HIV-1 RNA 为 100-10,000 拷贝/ml 的个体中和的 2 级病毒数量更高（分别为 p=< .0001 和 p=.076）。男性与广泛的 HIV-1 中和抗体活性相关 (p=.016)。这些结果表明，低但持续的 HIV 抗原表达与广泛的 HIV-1 中和抗体活性相关。在血浆病毒血症水平较高时，中和滴度降低。相反，在较低水平下，似乎没有足够的抗原刺激来维持高中和滴度。这些发现可能对进一步了解艾滋病毒感染的体液反应具有重要意义。
To examine the relationship between HIV-1 antigenic load (plasma RNA copies/ml) and broad HIV-1 neutralizing antibody activity. Plasma from 120 HIV-1 infected patients, including HIV-1 Natural Viral Suppressors (similar to Elite Controllers), was tested for neutralization against 15 Tier 1/Tier 2 HIV-1 pseudoviruses. Broad HIV-1 neutralizing antibody activity was confirmed with IgG and heterlogous clade testing (18 pseudoviruses from Clades A, C, and CRF02_AG). Statistical analysis was performed to determine factors associated with broad HIV-1 neutralizing antibody activity. Ten individuals with broad HIV-1 neutralizing antibody activity were identified. These individuals had a median CD4 count of 589 cells/ul (range 202–927), 1,611 HIV-1 RNA copies/ml (range 110–8,964), and 13 years since HIV diagnosis (range 1–22). There was a significant correlation between the presence of broadly neutralizing antibodies in those with HIV-1 RNA between 100 and 10,000 copies/ml compared to those <100 or >10,000 copies/ml (p=.0003 and .0245, respectively). Individuals with HIV-1 RNA 100–10,000 copies/ml had a higher number of Tier 2 viruses neutralized compared to the <100or >10,000 copies/ml groups (p=< .0001 and p=.076, respectively). Male sex was associated with broad HIV-1 neutralizing antibody activity (p=.016). These results indicate that low but persistent HIV antigen expression correlates with broad HIV-1 neutralizing antibody activity. At higher levels of plasma viremia, neutralization titers were diminished. Conversely, at lower levels, there appears to be insufficient antigen stimulation to maintain high neutralization titers. These findings may have important implications in furthering the understanding of the humoral response to HIV infection.