Monocytes from type 2 diabetic patients have a pro-inflammatory profile -: 1,25-Dihydroxyvitamin D3 works as anti-inflammatory
Monocytes from type 2 diabetic patients have a pro-inflammatory profile -: 1,25-Dihydroxyvitamin D3 works as anti-inflammatory
复制标题
DOI：
10.1016/j.diabres.2006.10.007
复制
发表时间：
2007-07-01
影响因子：
5.1
通讯作者：
Mathieu, Chantal
中科院分区：
文献类型：
--
作者：
Giulietti, Annapaula;van Etten, Evelyne;Mathieu, Chantal
导致 2 型糖尿病发病机制的确切因素仍然难以捉摸。最近，有人提出炎症和先天免疫系统的激活可能与 2 型糖尿病发病机制以及常见糖尿病并发症（主要是动脉粥样硬化）的发展有关。本研究的目的是研究单核细胞在这种亚临床炎症状态中的作用，并测试 1,25-二羟基维生素 D-3（维生素 D 的活性形式）作为抗炎剂的作用。为此，将来自 2 型糖尿病患者的单核细胞与来自健康对照和 1 型糖尿病患者的单核细胞进行了比较。通过定量实时 RT-PCR 测量新鲜分离和免疫刺激的单核细胞中炎症标志物的表达谱。与对照组和I型糖尿病患者相比，2型糖尿病患者的TNF-α、IL-6、IL-1、IL-8、COX-2、ICAM-1和B7-1的表达水平显着升高。 1,25-二羟基维生素 D3 能够下调 TNF-α、IL-6、IL-1 和 IL-8 的表达，证实了其免疫调节特性。根据这些数据，我们得出结论，2 型糖尿病患者的单核细胞具有促炎特性。此外，1,25-二羟基维生素 D3 能够调节这些单核细胞的炎症。 (c) 2006 Elsevier Ireland Ltd. 保留所有权利。
The exact factors contributing to the pathogenesis of type 2 diabetes remain elusive. Lately, it was suggested that inflammation and activation of the innate immune system could be linked to type 2 diabetes pathogenesis and also to the development of common diabetic complications, mainly atherosclerosis. The aim of this study was to investigate the role of monocytes in this sub-clinical inflammatory state and test 1,25-dihydroxyvitamin D-3, the active form of Vitamin D, as an anti-inflammatory agent. For this purpose, monocytes from type 2 diabetic patients were compared to monocytes from healthy controls and type 1 diabetic patients. The expression profile of inflammatory markers in freshly isolated and immune-stimulated monocytes was measured by quantitative real-time RT-PCR. Type 2 diabetic patients showed significantly higher expression levels of TNF-alpha, IL-6, IL-1, IL-8, COX-2, ICAM-1 and B7-1 compared to controls and type I diabetic patients. 1,25-Dihydroxyvitamin D3 was able to down-regulate the expression of TNF-alpha, IL-6, IL-1, and IL-8, confirming its immunomodulatory properties. From these data we concluded that monocytes from type 2 diabetic patients have a pro-inflammatory profile. In addition, 1,25-dihydroxyvitamin D3 was able to modulate inflammation in these monocytes. (c) 2006 Elsevier Ireland Ltd. All rights reserved.