Soil Abiotic Properties and Plant Functional Traits Mediate Associations Between Soil Microbial and Plant Communities During a Secondary Forest Succession on the Loess Plateau
Soil Abiotic Properties and Plant Functional Traits Mediate Associations Between Soil Microbial and Plant Communities During a Secondary Forest Succession on the Loess Plateau
复制标题
黄土高原次生林演替过程中土壤非生物特性和植物功能性状介导土壤微生物和植物群落之间的关联
DOI：
10.3389/fmicb.2019.00895
复制
发表时间：
2019-04-26
影响因子：
5.2
通讯作者：
Wang, Mao
中科院分区：
文献类型：
--
作者：
Chai, Yongfu;Cao, Ying;Wang, Mao
在次生林演替的背景下，地上-地下的相互作用被认为会影响植物群落的动态和功能结构。然而，在半干旱和干旱生态系统中，土壤微生物群落、土壤非生物特性、植物功能性状之间的联系尚不清楚。研究了黄土高原典型半干旱生态系统次生林演替≥150 a过程中土壤微生物物种多样性和群落组成的变化，及其对土壤非生物性质和植物功能性状的影响。植物群落片断被划分为6个演替阶段：废弃后1-4年、4-8年、8-15年、15-50年、50-100年和100-150年。通过对16 S rRNA基因的V4高变区和rRNA操纵子的内转录间隔区（ITS 2）区域进行高通量测序，分别分析了细菌和真菌群落。一个多变量的变异分配方法被用来估计所观察到的微生物群落组成的土壤性质和植物性状的贡献。我们发现相当大的差异，早期（S1-S3）和后期（S4-S6）演替阶段之间的细菌和真菌群落组成。总共有18个和12个独特的家庭，分别获得细菌和真菌，作为指标的微生物群落演替在六个阶段。细菌α多样性与植物物种α多样性呈正相关，真菌多样性与植物物种多样性呈负相关。某些真菌和细菌类群似乎与发生的优势植物物种在不同的演替阶段。土壤性质（pH、全N、全C、NH 4-N、NO3-N和PO 4-P浓度）和植物性状分别解释了细菌和真菌群落组成总变异的63.80%和56.68%。这些结果表明，土壤微生物群落与植物群落耦合，通过调解的微生物物种多样性和群落组成在一个长期的次生林演替在半干旱生态系统。根据土壤非生物特性和植物功能特性，细菌和真菌群落对植物群落演替的响应表现出不同的模式。
In the context of secondary forest succession, aboveground-belowground interactions are known to affect the dynamics and functional structure of plant communities. However, the links between soil microbial communities, soil abiotic properties, plant functional traits in the case of semi-arid and arid ecosystems, are unclear. In this study, we investigated the changes in soil microbial species diversity and community composition, and the corresponding effects of soil abiotic properties and plant functional traits, during a ≥150-year secondary forest succession on the Loess Plateau, which represents a typical semi-arid ecosystem in China. Plant community fragments were assigned to six successional stages: 1–4, 4–8, 8–15, 15–50, 50–100, and 100–150 years after abandonment. Bacterial and fungal communities were analyzed by high-throughput sequencing of the V4 hypervariable region of the 16S rRNA gene and the internal transcribed spacer (ITS2) region of the rRNA operon, respectively. A multivariate variation-partitioning approach was used to estimate the contributions of soil properties and plant traits to the observed microbial community composition. We found considerable differences in bacterial and fungal community compositions between the early (S1–S3) and later (S4–S6) successional stages. In total, 18 and 12 unique families were, respectively, obtained for bacteria and fungi, as indicators of microbial community succession across the six stages. Bacterial alpha diversity was positively correlated with plant species alpha diversity, while fungal diversity was negatively correlated with plant species diversity. Certain fungal and bacterial taxa appeared to be associated with the occurrence of dominant plant species at different successional stages. Soil properties (pH, total N, total C, NH4-N, NO3-N, and PO4-P concentrations) and plant traits explained 63.80% and 56.68% of total variance in bacterial and fungal community compositions, respectively. These results indicate that soil microbial communities are coupled with plant communities via the mediation of microbial species diversity and community composition over a long-term secondary forest succession in the semi-arid ecosystem. The bacterial and fungal communities show distinct patterns in response to plant community succession, according to both soil abiotic properties and plant functional traits.