Differential responses of rice to inoculation with wild-type and non-pathogenic mutants of Magnaporthe oryzae
Differential responses of rice to inoculation with wild-type and non-pathogenic mutants of Magnaporthe oryzae
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DOI：
10.1007/s11103-009-9495-9
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发表时间：
2009-08-01
影响因子：
5.1
通讯作者：
Minami, Eiichi
中科院分区：
文献类型：
--
作者：
Kato, Tomoaki;Tanabe, Shigeru;Minami, Eiichi
我们分析了水稻稻瘟病菌感染的反应，使用两个突变株缺乏Mgb 1和Mst 12，这是必不可少的附着胞和穿透钉的发展。这两个突变体诱导的植物抗毒素的积累水平比野生型低得多，这表明大量生产的植物抗毒素需要真菌入侵水稻细胞。H2 O2在单个全细胞中的强烈积累也需要真菌渗透。水稻基因表达微阵列分析显示了突变体特异的基因表达，表明水稻与M.在真菌侵入水稻细胞之前，水稻开始腐烂。过氧化物酶和β-1，3-葡聚糖酶的mRNA的原位检测表明，这些基因的表达也发生在渗透后，如观察到的植物抗毒素生产。
We analyzed the response of rice to Magnaporthe oryzae infection using two mutant strains deficient in Mgb1 and Mst12, which are essential for the development of appresoria and penetration pegs. Both mutants induced the much lower levels of accumulation of phytoalexins than wild-type, suggesting that the massive production of phytoalexins requires the fungal invasion of rice cells. Intense accumulation of H2O2 in a single whole cell also required fungal penetration. Microarray analysis of rice gene expression revealed mutant-specific gene expression, indicating that signal exchange between rice and M. oryzae commence before fungal penetration of the rice cell. In situ detection of mRNAs for peroxidase and beta-1,3-glucanase showed that expression of these genes also occurs after penetration as observed for phytoalexin production.