Detection of jun but not fos protein during developmental cell death in sympathetic neurons
Detection of jun but not fos protein during developmental cell death in sympathetic neurons
复制标题
交感神经元发育细胞死亡过程中检测到 jun 蛋白但未检测到 fos 蛋白
DOI：
--
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
C. Bell
中科院分区：
文献类型：
--
作者：
A. Messina;A. Jaworowski;C. Bell
在神经系统的正常发育过程中，大部分神经元通过称为细胞凋亡的主动过程死亡。我们计数了GH Wistar大鼠品系的上级颈神经节中的神经元和凋亡神经元的总数，该品系具有导致过度围产期细胞死亡的神经营养缺陷，并且通过使用光学剥离器方法来量化出生后发育期间的凋亡程度，来计数其正常对应物（N）中的神经元和凋亡神经元的总数。出生后第3和14天之间的神经元总数下降了10和40%，分别在N和GH。在GH神经节，1.5%的神经元凋亡在任何给定的时间，作为确定的存在下凝聚的染色质团块。某些类型的细胞死亡与即刻早期基因c-fos和c-jun的表达有关。因此，我们使用组织学和免疫细胞化学技术来检测单个神经元，并检测发育细胞死亡期间这些立即早期基因的产物。所有凋亡细胞的c-jun蛋白免疫阳性，而没有c-jun蛋白在非凋亡细胞检测。相反，在60%的非凋亡细胞的细胞核中检测到转录因子fos家族的成员，但只有一小部分细胞发生凋亡。这些结果表明，c-jun发生在致力于死亡的神经元中。这是第一次发现jun蛋白的存在与单个凋亡神经元中的正常程序性细胞死亡相关。© 1996 Wiley利斯公司
A large proportion of neurons die during normal development of the nervous system via an active process known as apoptosis. We counted the total number of neurons and apoptotic neurons in the superior cervical ganglion of the GH Wistar rat strain, which possesses a neurotrophic deficit leading to excessive perinatal cell death, and in its normal counterpart (N) by using the optical disector method to quantify the extent of apoptosis during postnatal development. Total neuron numbers fell between postnatal days 3 and 14 by 10 and 40% in N and GH, respectively. In GH ganglia, 1.5% of neurons were apoptotic at any given time, as determined by the presence of condensed chromatin clumps. Some types of cell death have been associated with expression of the immediate‐early genes c‐fos and c‐jun. Therefore, we used histological and immunocytochemical techniques to characterise individual neurons and to detect the products of these immediate‐early genes during developmental cell death. All apoptotic cells were immunopositive for c‐jun protein, whereas no c‐jun protein was detected in nonapoptotic cells. Conversely, members of the fos family of transcription factors were detected in the nucleus of 60% of nonapoptotic cells but in only a minor proportion of cells undergoing apoptosis. These results indicate that c‐jun occurs in neurons that are committed to die. This is the first situation in which the presence of jun protein has been correlated with normal programmed cell death in individual apoptotic neurons. © 1996 Wiley‐Liss, Inc.