Murine tongue muscle displays a distinct developmental profile of MRF and contractile gene expression.
Murine tongue muscle displays a distinct developmental profile of MRF and contractile gene expression.
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DOI：
10.1387/ijdb.10213080
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
Kirsten R. Dalrymple;Theodore I. Prigozy;Mark Mayo;Lawrence H. Kedes;C. F. Shuler
中科院分区：
文献类型：
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作者：
Kirsten R. Dalrymple;Theodore I. Prigozy;Mark Mayo;Lawrence H. Kedes;C. F. Shuler
很少有研究涉及存在于人体肌肉和颅面器官之间的分子差异。在这项研究中，我们描述了与舌头肌肉组织的决定和分化相关的分子事件。我们评估了肌生成调节因子的表达以及发育调节的肌球蛋白重链（MHC），作为分化标记的基因。这些结果表明舌肌和肢体肌肉是通过不同的分子途径形成的。在成肌细胞的决定过程中，参与舌形成的成肌细胞优先表达Myf-5而不是MyoD。随后，分离的肌原素表达区标志着首先是小的原发性肌纤维的分化，然后是较大的继发性肌纤维的分化。分化标记分析表明，与身体肌肉相比，舌肌也具有独特的MHC表达谱。不同于发育肢体的成肌细胞表达胚胎和新生儿形式的MHC，随后表达MHC-慢型，舌肌细胞从孕龄E12开始共同表达MHC-胚胎、MHC-慢型和MHC-快型同种异构体。MHC胚胎亚型和MHC快速亚型的蛋白几乎同时检测到。有趣的是，在任何发育阶段，MHC慢转录物似乎都不会被翻译成可检测的MHC慢蛋白。这些结果提供了进一步的证据，表明舌骨骼肌代表的成肌细胞谱系与肢体发育不同。
Few studies have addressed the molecular differences that exist between muscles of the body and those of the craniofacial apparatus. In this study, we characterize the molecular events associated with determination and differentiation of the tongue musculature. We assess the expression of myogenic regulatory factors as well as the developmentally regulated myosin heavy chain, (MHC), genes which serve as markers of differentiation. These results suggest that tongue and limb muscle form by distinct molecular pathways. The myoblasts that contribute to the formation of the tongue preferentially express Myf-5 during myoblast determination rather than MyoD. Subsequently, isolated regions of myogenin expression mark the differentiation of first, the small primary myofibers and later, the larger secondary myofibers. Analysis of differentiation markers demonstrates that the tongue muscle also assumes a unique profile of MHC expression as compared to that of the muscles of the body. Unlike the myoblasts of the developing limb, which express embryonic and neonatal forms of MHC and later express MHC-slow, the tongue myoblasts co-express MHC-embryonic, MHC-slow and MHC-fast isoforms from gestational age E12. Proteins for MHC embryonic and MHC fast isoforms are detected almost simultaneously. Interestingly, MHC-slow transcripts do not appear to be translated into a detectable MHC slow protein at any developmental stage assayed. These results provide further evidence to suggest that skeletal tongue muscle represents a myoblast lineage that develops differently than the limb.