Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection
Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection
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DOI：
10.1002/lsm.20415
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发表时间：
2007-01-01
影响因子：
2.4
通讯作者：
Hamblin, Michael R.
中科院分区：
文献类型：
--
作者：
Garcez, Aguinaldo S.;Ribeiro, Martha S.;Hamblin, Michael R.
背景与目的：比较抗菌光动力治疗（PDT）、标准根管治疗和联合治疗消除感染根管细菌生物膜的效果。研究设计/材料与方法：用稳定的生物发光革兰氏阴性菌、奇异变形杆菌和铜绿假单胞菌接种10颗刚拔出的人单根牙齿，在制备好的根管内形成3 d的生物膜。生物荧光成像用于连续量化细菌负荷。PDT采用聚乙烯亚胺与氯胺（e6）的缀合物作为光敏剂（PS），通过200亩光纤将660 nm二极管激光送入根管，并与机械清创和消毒冲洗的标准根管治疗进行比较和结合。结果：根管治疗可使细菌生物发光降低90%，而PDT治疗可使细菌生物发光降低95%。联合用药使生物发光降低了98%，重要的是，联合用药24小时后观察到的细菌再生比单独用药少得多（P < 0.0005）。结论：生物发光显像是监测根管治疗的有效方法。抗菌PDT可能在优化牙髓治疗中发挥作用。
Background and Objective: To compare the effectiveness of antimicrobial photodynamic therapy (PDT), standard endodontic treatment and the combined treatment to eliminate bacterial biofilms present in infected root canals.Study Design/Materials and Methods: Ten single-rooted freshly extracted human teeth were inoculated with stable bioluminescent Gram-negative bacteria, Proteus mirabilis and Pseudomonas aeruginosa to form 3-day biofilms in prepared root canals. Biolumineseence imaging was used to serially quantify bacterial burdens. PDT employed a conjugate between polyethylenimine and chlorin(e6) as the photosensitizer (PS) and 660-nm diode laser light delivered into the root canal via a 200-mu fiber, and this was compared and combined with standard endodontie treatment using mechanical debridement and antiseptic irrigation.Results: Endodontic therapy alone reduced bacterial bioluminescence by 90% while PDT alone reduced bioluminescence by 95%. The combination reduced bioluminescence by >98%, and importantly the bacterial regrowth observed 24 hours after treatment was much less for the combination (P < 0.0005) than for either single treatment.Conclusions: Bioluminescence imaging is an efficient way to monitor endodontic therapy. Antimicrobial PDT may have a role to play in optimized endodontic therapy.