[Effects of Gualou Xiebai Banxia decoction on blood lipid content, oxidative stress and ox-LDL/Lox-1 pathway in ApoE-/- mice].
[Effects of Gualou Xiebai Banxia decoction on blood lipid content, oxidative stress and ox-LDL/Lox-1 pathway in ApoE-/- mice].
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DOI：
10.19540/j.cnki.cjcmm.20161222.076
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发表时间：
2017-02-01
期刊：
影响因子：
--
通讯作者：
Li, Jing-Hua
中科院分区：
文献类型：
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作者：
Guo, Jian-En;Mi, Shu-Bin;Li, Jing-Hua
观察瓜蒌薤白半夏汤调节脂代谢、抗氧化及干预ox-LDL/Lox-1通路的作用，探讨其抗动脉粥样硬化（AS）的作用机制。采用42只Apo-E-/-雄性小鼠高脂饮食建立AS模型。将AS模型小鼠随机分为模型组、辛伐他汀组、甘心痹汤高、低剂量组。以C57 BL/6 J雄性小鼠为正常对照组，n=10，治疗8周。末次给药后24 h检测血清TC、TG、LDL-C、HDL-C、SOD、MDA、GSH-px、ox-LDL水平。HE染色观察主动脉组织结构变化;结果表明，模型组大鼠血脂水平、血清MDA、ox-LDL水平均显著高于正常对照组，而SOD、MDA、ox-LDL水平显著高于正常对照组，GSH-px水平明显低于正常对照组，Lox-1蛋白和mRNA表达水平也明显高于对照组（P
To observe the functions of Gualou Xiebai Banxia decoction（GXBD） on regulating lipid metabolism, anti-oxidation, and interposing ox-LDL/Lox-1 pathway, and to explore its anti-atherosclerosis (AS) mechanisms. AS models were established by using 42 Apo-E-/- male mice with high fat diet. AS model mice were randomly divided into the model group, simvastatin group, and GXBD high and low dose groups. C57BL/6J male mice were used as the normal control group, n=10 and the treatment lasted for 8 weeks. The levels of TC, TG, LDL-C, HDL-C, SOD, MDA, GSH-px, and ox-LDL in blood serum were tested 24 h after the last administration. The changes of aortic tissues structure were observed by HE staining; the expression levels of Lox-1 protein and the expression levels of mRNA were detected by Western blot and PCR respectively.Results showed that the blood lipid levels and MDA, ox-LDL levels in blood serum of model group were significantly higher than those in the normal control group, but SOD, GSH-px levels were significantly lower than those in the normal control group, and the Lox-1 protein and mRNA expression levels were also significantly higher than those in the control group(P