Periostin in the bronchial lavage fluid of asthma patients
Periostin in the bronchial lavage fluid of asthma patients
复制标题
哮喘患者支气管灌洗液中的骨膜素
DOI：
10.1016/j.alit.2015.01.001
复制
发表时间：
2015
影响因子：
6.8
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
akamura;Y.;Nagashima;H.;Ohta;S.;Ono;J.;Yamauchi;K. & Izuhara;K.
血清骨膜蛋白最近成为支气管哮喘的一种新的和有用的生物标志物。1血清骨膜蛋白是反映2型免疫应答的替代生物标志物，因为骨膜蛋白是白细胞介素-4（IL-4）或IL-13（2型免疫应答的标志性细胞因子）的下游分子。骨膜蛋白的这一特性可以潜在地应用于预测拮抗剂如抗IL-13或抗IgE抗体针对2型免疫应答的功效。此外，我们最近发现，由于骨膜蛋白反映了支气管哮喘的组织重塑或纤维化，它可以预测哮喘患者对吸入皮质类固醇的低反应性，特别是在那些晚发型和嗜酸性粒细胞占主导地位的患者中。尽管骨膜蛋白作为支气管哮喘的生物标志物是有用的，但血清骨膜蛋白可受到肺组织以外部位（特别是骨）产生的骨膜蛋白的影响。因此，如果能够特异性地检测肺组织中的骨膜蛋白，将对哮喘患者的护理和治疗大有裨益。然而，骨膜蛋白如何从细胞中分泌并在哮喘患者中循环仍然不清楚。尚未进行研究以调查骨膜蛋白是否在哮喘患者的支气管腔内分泌。支气管驻留细胞、上皮细胞、成纤维细胞和内皮细胞被认为是肺组织中骨膜蛋白的来源。4 e6已经在体外证明，在支气管上皮细胞中，骨膜蛋白可以从基底侧分泌，但不能从顶侧分泌。4这些结果否定了骨膜蛋白在哮喘患者的支气管腔内分泌的观点。相反，据报道，骨膜蛋白在慢性鼻窦炎患者的鼻灌洗液中高度检测，这可能仍然支持这一观点。因此，在本研究中，我们检测了哮喘患者支气管灌洗液（BLF）中的骨膜蛋白，以探讨其是否可以作为支气管哮喘的更特异性生物标志物。
Serum periostin has recently emerged as a novel and useful biomarker for bronchial asthma. 1 Serum periostin is a surrogate biomarker reflecting type 2 immune responses because periostin is a downstream molecule of interleukin-4 (IL-4) or IL-13, the signature cytokines of type 2 immune responses. This characteristic of periostin can potentially be applied to predict the efficacy of antagonists such as anti-IL-13 or anti-IgE antibodies against type 2 immune responses. Furthermore, we have recently found that since periostin reflects tissue remodeling or fibrosis in bronchial asthma, it can predict hypo-responsiveness to inhaled corticosteroids in asthma patients, particularly in those who are late-onset and eosinophil-dominant. 2, 3In spite of the usefulness of periostin as a biomarker for bronchial asthma, serum periostin can be affected by periostin produced in sites other than lung tissues, particularly bones. Therefore, it would be of great benefit in caring for and treating asthma patients if periostin in lung tissues could be specifically detected. However, it remains obscure how periostin is secreted from the cells and circulated in asthma patients. No study has yet been performed to investigate whether periostin is secreted in the bronchial lumens of asthma patients. Bronchial resident cellsdepithelial cells, fibroblasts, and endothelial cellsdare assumed to be sources of periostin in lung tissues. 4 e6 It has been demonstrated in vitro that periostin can be secreted from the basal side, but not from the apical side, in bronchial epithelial cells. 4 These results negate the notion that periostin is secreted in the bronchial lumens in asthma patients. In contrast, it has been reported that periostin is highly detected in nasal lavage fluid in chronic rhinosinusitis patients, which may nevertheless support this notion. 7 Therefore, in this study, we examined periostin in the bronchial lavage fluid (BLF) of asthma patients to explore whether it can be a more specific biomarker for bronchial asthma.