STUDY ON THE MUTAGENIC ACTIVITY OF 13 BIOFLAVONOIDS WITH THE SALMONELLA ARA TEST
STUDY ON THE MUTAGENIC ACTIVITY OF 13 BIOFLAVONOIDS WITH THE SALMONELLA ARA TEST
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DOI：
10.1093/mutage/6.4.289
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发表时间：
1991-07-01
期刊：
影响因子：
2.7
通讯作者：
PUEYO, C
中科院分区：
文献类型：
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作者：
JURADO, J;ALEJANDREDURAN, E;PUEYO, C
用鼠伤寒沙门氏菌L-阿拉伯糖正向突变试验研究了13种黄酮类化合物的致突变性。在哺乳动物代谢激活(S9混合物)存在或不存在的情况下，通过平板掺入和预孵化突变方案对每种类黄酮进行测试。所有的黄酮类化合物都给出了剂量-反应关系，并诱导了一些被认为具有统计学意义的Ara(R)突变。在Ara试验中，它们的最小诱变剂量(MMD)有很大不同，覆盖了400倍的范围：从槲皮素的4nmo1到紫杉酯的1626nmo1。黄酮醇是最强的诱变剂，其致突变能力(MMD-1)约为槲皮素的27%至2%。相比之下，其他类黄酮类化合物的致突变性只占不到或等于1%。本文报道的Ara正向突变试验数据表明，与以前报道的His反向突变试验一样，生物黄酮类化合物的结构要求是：(I)3位自由羟基的黄酮醇是最强的诱变黄酮类化合物，(Ii)2，3双键的饱和降低了诱变能力，(Iii)3‘和4’位的游离羟基影响代谢激活的非要求。在Ara测试中，槲皮素和芦丁的诱变特性支持这样的观点，即这些黄酮醇不是葡萄酒等复杂混合物中的主要诱变剂。
The mutagenicity of 13 flavonoids has been investigated with the L-arabinose forward mutation assay of Salmonella typhimurium. Each flavonoid was tested by both plate incorporation and preincubation mutagenesis protocols in the presence or the absence of mammalian metabolic activation (S9 mixture). All flavonoids gave a dose - response relationship and induced a number of Ara(R) mutants considered statistically significant. Their minimum mutagenic doses (MMD) differed markedly in the Ara test, covering a 400-fold range: from 4 nmol for quercetin to 1626 nmol for taxifolin. Flavonols were the strongest mutagens, with mutagenic potencies (MMD-1) representing from 27 to approximately 2% that of quercetin. Comparatively, the mutagenicities of other flavonoids represented only less-than-or-equal-to 1%. The data reported in this paper for the Ara forward mutation test suggest structural requirements for mutagenicity of bioflavonoids like those previously reported for the His reverse mutation assay: (i) flavonols with a free hydroxyl at position 3 are the strongest mutagenic flavonoids, (ii) saturation of the 2,3 double bond diminishes the mutagenic potency, and (iii) free hydroxyl groups at positions 3' and 4' influence the non-requirement for metabolic activation. The mutagenic properties of quercetin and rutin in the Ara test support the idea that these flavonols are not the major putative mutagens in complex mixtures such as wine.