Comparison of Antioxidant Activity of α-, β-Carotene, Lutein and Lycopene by High Pressure Liquid Chromatography
Comparison of Antioxidant Activity of α-, β-Carotene, Lutein and Lycopene by High Pressure Liquid Chromatography
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高压液相色谱法比较α-、β-胡萝卜素、叶黄素和番茄红素的抗氧化活性
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
Hye Young Kim
中科院分区：
文献类型：
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作者：
Hye Young Kim
建立了一种测定脂质过氧化体系中丙二醛含量的新的HPLC方法，并通过新开发的HPLC分析和TBA值比较了-，-胡萝卜素、叶黄素和番茄红素的抗氧化活性。此外，测定了大鼠肝微粒体在不同解冻次数下的丙二醛形成能力。结果表明，丙二醛在5.60 min内被洗脱，标准曲线峰面积与浓度呈线性关系。随着解冻次数的增加，微粒体中MA含量下降，这可能是由于细胞膜的破坏。在Fe+3-ADP/NADPH和百草枯/NADPH体系中，番茄红素、叶黄素和β-胡萝卜素的抗氧化活性均强于DL--生育酚的β-胡萝卜素。TBA值和HPLC法测定的丙二醛含量表明，类胡萝卜素和DL-生育酚对Fe+3-ADP/NADPH脂质过氧化体系的抑制作用相似。
A new HPLC method for determining malonaldehyde content in lipid peroxidation systems was developed and antioxidant activities of -, - carotene, lutein and lycopene were compared by a newly developed HPLC analysis and by TBA value. In addition, malonaldehyde forming ability of rat liver microsome was determined depending on thawing numbers. As results, malonaldehyde was eluted at a retention o f5.60 min and showed a linear relationship between peak area and concentration in standard curve. The MA content of microsome decreased with thawing numbers possible by destruction of cellular membranes. Lycopene, lutein and -carotene showed stronger antioxidant activities than -carotene of DL--tocopherol both in Fe+3-ADP/NADPH and in paraquat/NADPH system. The inhibitory effects of carotenoids and DL--tocopherol on Fe+3-ADP/NADPH lipid peroxidation system was similar by TBA value and by the HPLC analysis for malonaldehyde.